In green plants H₂O is the hydrogen donor and is oxidised to O₂. Some organisms do not release O₂ during photosynthesis. When H₂S, instead is the hydrogen donor for purple and green sulphur bacteria, the 'oxidation' product is sulphur or sulphate depending on the organism and not O₂. Hence, he inferred that the O₂ evolved by the green plant comes from H₂O, not from carbon dioxide. This was later proved by using radioisotopic techniques. The correct equation, that would represent the overall process of photosynthesis is therefore:
Choose the correct sequence of discoveries: A. Role of chlorophyll B. Oxygen comes from H₂O C. Sunlight is essential D. Starch formation as evidence
Correct answer: B — D → C → A → B
Starch formation (Sachs) → Sunlight essential (Ingenhousz) → Role of chlorophyll (Sachs) → Oxygen from H₂O (van Niel).
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