Class 11 · Photosynthesis in Higher Plants

ATP and NADPH Requirements in Calvin Cycle — NEET Biology

✅ Asked in NEET 2026
✅ NEET 2026 PYQ · Asked 4 times

How many ATP and NADPH molecules are required to make one molecule of glucose through the Calvin pathway?

Q1 of 4NEET 2026 (cancelled)

How many ATP and NADPH molecules are required to make one molecule of glucose through the Calvin pathway?

Q2 of 4NEET 2024

(NEET 2024) How many molecules of ATP and NADPH are required for every molecule of CO₂ fixed in the Calvin cycle?

Q3 of 4NEET 2024

(NEET 2024) How many molecules of ATP and NADPH are required, respectively, for fixation of every CO₂ molecule entering the Calvin cycle?

Q4 of 4NEET 2023

How many ATP and NADPH₂ are required for the synthesis of one molecule of glucose during Calvin cycle? (NEET 2023)

Answer & NCERT explanation

Correct answer: A 18 ATP and 12 NADPH

Each Calvin cycle turn fixes 1 CO2 using 3 ATP and 2 NADPH. To make one glucose (6 carbons), 6 turns are needed: 6x3 = 18 ATP and 6x2 = 12 NADPH.

Read more NCERT concept on the PYQ

📖 NCERT Source

Hence for every CO₂ molecule entering the Calvin cycle, 3 molecules of ATP and 2 of NADPH are required. It is probably to meet this difference in number of ATP and NADPH used in the dark reaction that the cyclic phosphorylation takes place.

NCERT Biology · Class 11 · Chapter 11 · Paragraph 59
How NTA Uses This Concept

For every one CO₂ molecule fixed in the Calvin cycle, exactly 3 ATP and 2 NADPH molecules are consumed. The NTA tests whether students can recall these specific stoichiometric ratios and understand why more ATP than NADPH is needed. Students commonly confuse the numbers or forget that cyclic photophosphorylation specifically occurs to generate the extra ATP molecules required. The key to remembering: 3 ATP per CO₂ (not 2), and 2 NADPH per CO₂. This imbalance explains why cyclic photophosphorylation is essential—it produces additional ATP without generating NADPH, balancing the energy requirements of the dark reaction.

❓ Frequently Asked Questions
What does NCERT say about Hence for every CO?
Hence for every CO₂ molecule entering the Calvin cycle, 3 molecules of ATP and 2 of NADPH are required. It is probably to meet this difference in number of ATP and NADPH used in the dark reaction that the cyclic phosphorylation takes place.
Has this concept appeared in NEET?
Yes — appeared in NEET 2026 (cancelled), 2024, 2024, 2023. States exactly 3 ATP and 2 NADPH required per CO2 in Calvin cycle
Which chapter is this from?
Photosynthesis in Higher Plants, Class 11 NCERT Biology.

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