Gene Therapy: Mechanism and Vectors — NEET Biology
📚 Practice ConceptGene therapy is the insertion of genes into an individual's cells and tissues to treat diseases especially hereditary diseases. It does so by replacing a defective mutant allele with a functional one or gene targeting which involves gene amplification. Viruses that attack their hosts and introduce their genetic material into the host cell as part of their replication cycle are used as vectors to transfer healthy genes or more recently portions of genes.
Match the following molecular techniques with their primary functions or principles: Column I (Technique) (P) Polymerase Chain Reaction (PCR) (Q) Enzyme Linked Immuno-sorbent Assay (ELISA) (R) Autoradiography with a radioactive probe (S) Gene Therapy Column II (Function/Principle) (i) Detection of low pathogen load or genetic mutations by nucleic acid amplification. (ii) Treatment of genetic defects by inserting functional genes into target cells/tissues. (iii) Detection of mutated genes in cell clones by observing the absence of probe hybridization. (iv) Pathogen detection based on specific antigen-antibody interactions.
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