Year-wise Weightage (2015–2026)

2026 vs 2025: +2 more questions vs 2025

YearQuestions AskedMarks
2026312
202514
202428
2023416
202228
Show all years (2015–2021)
2021624
2020312
2019520
2018416
201714
2016416
2015
Q1
NEET 2016 Phase 1

Which part of the tobacco plant is infected by Meloidogyne incognita?

Q2
NEET 2016 Phase 1 · NEET 2017 · NEET 2019 · NEET 2019 Odisha · NEET 2020 · NEET 2021 · NEET 2023 Phase 2

Which of the following statements is not correct?

Q3
NEET 2016 Phase 1

The two polypeptides of human insulin are linked together by:

Q4
NEET 2016 Phase 2

Which kind of therapy was given in 1990 to a four-year-old girl with adenosine deaminase (ADA) deficiency? (NEET 2016 (Phase 2))

Q5
NEET 2018

Use of bioresources by multinational companies and organisations without authorisation from the concerned country and its people is called (NEET 2018)

Q6
NEET 2018

Which of the following is commonly used as a vector for introducing a DNA fragment in human lymphocytes? (NEET 2018)

Q7
NEET 2018

A ‘new’ variety of rice was patented by a foreign company, though such varieties have been present in India for a long time. This is related to (NEET 2018)

Q8
NEET 2018

In India, the organisation responsible for assessing the safety of introducing genetically modified organisms for public use is (NEET 2018)

Q9
NEET 2019

What triggers activation of protoxin to active Bt toxin of Bacillus thuringiensis in boll worm? (NEET 2019)

Q10
NEET 2019

Which of the following is true for Golden rice?

Q11
NEET 2019 Odisha

In RNAi, the genes are silenced using: (NEET 2019 (Odisha))

Q12
NEET 2019

Which of the following features of genetic code does allow bacteria to produce human insulin by recombinant DNA technology? (NEET 2019)

Q13
NEET 2020

Bt cotton variety that was developed by the introduction toxin gene of Bacillus thuringiensis (bt) is resistant to:

Q14
NEET 2020

Match the following columns and select the correct option. Column-I (a) Bt cotton (b) Adenosine deaminase deficiency (c) RNAi (d) PCR Column-II (i) Gene therapy (ii) Cellular defence (iii) Detection of HIV infection (iv) Bacillus thuringiensis (NEET 2020)

Q15
NEET 2021

With regard to insulin choose correct options. (a) C-peptide is not present in mature insulin. (b) The insulin produced by rDNA technology has C-peptide. (c) The pro-insulin has C-peptide. (d) A-peptide and B-peptide of insulin are interconnected by disulphide bridges Choose the correct answer from the options given below. (NEET 2021)

Q16
NEET 2021

Now a days it is possible to detect the mutated gene causing cancer by allowing radioactive probe to hybridise its complementary DNA in a clone of cells, followed by its detection using autoradiography because : (NEET 2021)

Q17
NEET 2021

When gene targetting involving gene amplification is attempted in an individual's tissue to treat disease, it is known as: (NEET 2021)

Q18
NEET 2021

The Adenosine deaminase deficiency results into:

Q19
NEET 2021

For effective treatment of the disease, early diagnosis and understanding its pathophysiology is very important. Which of the following molecular diagnostic techniques is very useful for early detection? (NEET 2021)

Q20
NEET 2022

Statements related to human insulin are given below: Which statement(s) is/are correct about genetically engineered Insulin? (a) Pro-hormone insulin contain extra stretch of C-peptide (b) A peptide and B-peptide chains of insulin were produced separately in E. coli, extracted and combined by creating a disulphide bond between them. (c) Insulin used for treating Diabetes was extracted from Cattles and Pigs. (d) Pro-hormone insulin needs to be processed for converting into a mature and functional hormone. (e) Some patients develop allergic reactions to the foreign insulin. Choose the most appropriate answer from the options given below: (NEET 2022)

Q21
NEET 2022

In gene therapy of Adenosine Deaminase (ADA) deficiency, the patient requires periodic infusion of genetically engineered lymphocytes because: (NEET 2022)

Q22
NEET 2023 Phase 1

Which one of the following techniques does not serve the purpose of early diagnosis of a disease for its early treatment? (NEET 2023)

Q23
NEET 2023 Phase 2

Select incorrect statement, regarding chemical structure of insulin. (NEET 2023 (Phase 2))

Q24
NEET 2023 Phase 2

Match List - I with List - II. List - I (A) Gene therapy (B) RNA interference (C) ELISA (D) Gel Electrophoresis List - II (I) Separation of DNA fragments (II) Diagnostic test for AIDS (III) Cellular defence (IV) Allows correction of a gene defect Choose the correct answer from the options given below : (NEET 2023 (Phase 2))

Q25
NEET 2024

Given below are two statements: Statement I: Bt toxins are insect group specific and coded by a gene cry 1Ac. Statement II: Bt toxin exists as inactive protoxin in B. thuringiensis. However, after ingestion by the insect the inactive protoxin gets converted into active form due to acidic pH of the insect gut. In the light of the above statements, choose the correct answer from the options given below: (NEET 2024)

Q26
NEET 2024

Match List I with List II. List I A. α-1 antitrypsin B. Cry 1Ab C. Cry 1Ac D. Enzyme replacement therapy List II I. Cotton bollworm II. ADA deficiency III. Emphysema IV. Corn borer Choose the correct answer from the options given below. (NEET 2024)

Q27
NEET 2025

Which of the following genetically engineered organisms was used by Eli Lilly to prepare human insulin? (NEET 2025)

Q28
NEET 2026 (1)

The human protein named α-1-antitrypsin, obtained from transgenic animals, is used for the treatment of ______.

Q29
ReNEET 2026

The inactive form of Bt toxin is converted to the active form in the insect gut _______.

Q30
NEET 2026 (1)

The toxin proteins isolated from Bacillus thuringiensis, coded by which of the following genes would control cotton bollworms and corn borer, respectively? (NEET 2026)

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Biotechnology and Its Applications — NEET PYQ Analysis

Biotechnology and Its Applications is a Class 12 Botany chapter that has been consistently tested in NEET from 2016 to 2026. This page contains 30 authentic previous year questions from actual NEET exam papers — not model questions or predictions.

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Study Biotechnology and Its Applications Concept-by-Concept

Every NCERT paragraph from Biotechnology and Its Applications that NEET has tested. Each is its own concept page with the question, answer and exam-pattern analysis. Ranked by how many times NEET has tested the paragraph.

View all 18 concepts from Biotechnology and Its Applications