The taq polymerase enzyme is obtained from:
Stirred-tank bioreactors have been designed for
Which of the following restriction enzymes produces blunt ends?
Which of the following is not a component of downstream processing?
Which of the following is not a feature of the plasmids?
A foreign DNA and plasmid cut by the same restriction endonuclease can be joined to form a recombinant plasmid using
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Which of the following is a restriction endonuclease?
A gene whose expression helps to identify transformed cell is known as
The process of separation and purification of expressed protein before marketing is called
What is the criterion for DNA fragments movement on agarose gel during gel electrophoresis?
Which of the following is commonly used as a vector for introducing a DNA fragment in human lymphocytes?
The correct order of steps in Polymerase Chain Reaction (PCR) is
Given below are four statements pertaining to separation of DNA fragments using gel electrophoresis. Identify the incorrect statements. (a) DNA is negatively charged molecule and so it is loaded on gel towards the Anode terminal (b) DNA fragments travel along the surface of the gel whose concentration does not affect movement of DNA. (c) Smaller the size of DNA fragment larger is the distance it travels through it. (d) Pure DNA can be visualized directly by exposing UV radiation. Choose correct answer from the options given below
Which one of the following equipments is essentially required for growing microbes on a large scale, for industrial production of enzymes?
Match the following enzymes with their functions : (a) Restriction endonuclease (i) Joins the DNA fragements (b) Restriction exonuclease (ii) Extends primers on genomic DNA template (c) DNA ligase (iii) Cuts DNA at specific position (d) Taq polymerase (iv) Removes nucleotides from the ends of DNA Select the correct option from the following
Following statements describe the characteristics of the enzyme Restriction Endonuclease. Identify the incorrect statement.
An enzyme catalysing the removel of nucleotides from ends of DNA is:
DNA precipitation out of a mixture of biomolecules can be achieved by treatment with
The two antibiotic resistance genes on vector pBR322 are for
Which or the following statements is incorrect?
A selectable marker is used to:
In gel electrophoresis, separated DNA fragments can be visualized with the help of
Identify the wrong statement with regard to Restriction Enzymes.
Choose the correct pair from the following:
The specific palindromic sequence which is recognized by EcoRI is
Match the organism with its use in biotechnology (a) Bacillus (b) Thermus aquaticus (c) Agrobacterium tumefaciens (d) Salmonella typhimurium (i) Cloning vector (ii) Construction of first rDNA (iii) DNA polymerase (iv) Cry proteins Select the correct option from the following:
The sequence that controls the copy number of the linked DNA in the vector is termed
Which of the following is a correct sequence of steps in a PCR (Polymerase Chain Reaction) ?
Which of the following is not an application of PCR (Polymerase Chain Reaction)
Plasmid pBR322 has PsI restriction enzyme site within gene ampR that confers ampicillin resistance. If this enzyme is used for inserting a gene for β-galactoside production and the recombinant plasmid is inserted in a E.coli strain
A specific recognition sequence identified by endonucleases to make cuts at specific positions within the DNA is:
During the purification process for recombinant DNA technology, addition of chilled ethanol precipitates out
Which of the following is not a desirable feature of a cloning vector ?
Given below are two statements: Statement I: Restriction endonucleases recognise specific sequences to cut DNA known as palindromic nucleotide sequences. Statement II: Restriction endonucleases cut the DNA strand a little away from the centre of the palindromic site. In the light of the above statements, choose the most appropriate answer from the options given below:
Which one of the following statements is not true regarding gel electrophoresis technique?
Given below are two statements: one is labelled as Assertion (A) and the other is labelled as Reason (R). Assertion (A): Polymerase chain reaction is used in DNA amplification. Reason (R): The ampicillin resistant gene is used as a selectable marker to check transformation. In the light of the above statements, choose the correct answer from the options given below:
In the following palindromic base sequence of DNA, which one can be cut easily by a particular restriction enzyme?
Match List-I with List-II. List-I List-II (A) Kanamycin (I) Delivers genes into animal cells (B) ClaI (II) Selectable marker (C) Disarmed retroviruses (III) Restriction site (D) Kanamycin R gene (IV) Antibiotic resistance Choose the correct answer from the options given below.
Which of the following is not a cloning vector?
Main steps in the formation of Recombinant DNA are given below. Arrange these steps in a correct sequence. A. Insertion of recombinant DNA into the host cell. B. Cutting of DNA at specific location by restriction enzyme. C. Isolation of desired DNA fragment. D. Amplification of gene of interest using PCR. Choose the correct answer from the options given below:
Which of the following can act as molecular scissors?
Which of the following statement is incorrect about Agrobacterium tumifaciens?
Upon exposure to UV radiation, DNA stained with ethidium bromide will show
Thermostable DNA polymerase used in PCR was isolated from :
The "Ti plasmid" of Agrobacterium tumefaciens stands for
The following diagram showing restriction sites in E coli cloning vector pBR322. Find the role of 'X' and 'Y' genes:
In the above represented plasmid an alien piece of DNA is inserted at EcoRI site. Which of the following strategies will be chosen to select the recombinant colonies?
Polymerase chain reaction (PCR) amplifies DNA following the equation
Given below are two statements : Statement I : The DNA fragments extracted from gel electrophoresis can be used in construction of recombinant DNA. Statement II : Smaller size DNA fragments are observed near anode while larger fragments are found near the wells in an agarose gel. In the light of the above statements, choose the most appropriate answer from the options given below :
Which of the following enzyme(s) are NOT essential for gene cloning? A. Restriction enzymes B. DNA ligase C. DNA mutase D. DNA recombinase E. DNA polymerase Choose the correct answer from the options given below :
The blue and white selectable markers have been developed which differentiate recombinant colonies from non-recombinant colonies on the basis of their ability to produce colour in the presence of a chromogenic substrate. Given below are two statements about this method: Statement I : The blue coloured colonies have DNA insert in the plasmid and they are identified as recombinant colonies. Statement II : The colonies without blue colour have DNA insert in the plasmid and are identified as recombinant colonies. In the light of the above statements, choose the most appropriate answer from the options given below :
Assertion A: In recombinant DNA technology, lysozyme is used for disrupting bacterial cells while cellulase is used for plant cells. Reason R: Isolation of genetic material needs disruption of cells. Choose the most appropriate answer:
During PCR, primers bind to the DNA strands in the ______ step.
Which of the following statements are correct with respect to DNA separation, isolation and visualization? A. The cutting of DNA is done by molecular scissors. B. The DNA fragments separate according to their size in an agarose gel, upon electrophoresis. C. The separated DNA fragments can be seen without staining when exposed to UV light. D. The separated DNA fragments, when stained with ethidium bromide, can be seen in visible light. Choose the correct answer from the options given below:
Match List-I with List-II: List-I A. Transformation B. Cloning site C. Selection D. Ori List-II I. Restriction enzyme II. Transfer DNA to host bacteria III. Replication IV. Antibiotic Choose the correct answer from the options given below:
Statement I: Plasmids are autonomously replicating DNA. Statement II: Plasmids are extrachromosomal DNA. Choose the most appropriate answer:
Which of the following statements are not true regarding restriction endonucleases? A. They are called molecular scissors. B. These are the enzymes responsible for restricting the growth of bacteriophages in E. coli. C. They cut the DNA only at the centre of the palindromic sites. D. They remove nucleotides only from the ends of DNA fragments. E. They recognise specific palindromic base-pair sequences. Choose the answer from the options given below:
Insertion of a foreign DNA at BamHI site in an E.coli cloning vector pBR322 results in the loss of antibiotic resistance towards:
Match List I with List II: List-I A. Genetically modified organism B. Thermostable DNA polymerase C. Ti plasmid D. pBR322 List-II (I) Agrobacterium tumefaciens (II) Bt cotton (III) Thermus aquaticus (IV) Escherichia coli Choose the correct answer from the options given below:
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