Biotechnology: Principles and Processes

The first restriction endonuclease was:

In recombinant DNA technology, the cloning vector is:

PCR stands for:

Which of the following is used as a selectable marker in E. coli?

Gel electrophoresis is used for:

The role of DNA ligase in genetic engineering is:

Match List I with List II — A. Genetically modified organism, B. Thermostable DNA polymerase, C. Ti plasmid, D. pBR322 — with I. Agrobacterium tumefaciens, II. Bt cotton, III. Thermus aquaticus, IV. Escherichia coli ( NEET 2026)

Identify the correct sequence of steps in each cycle of Polymerase Chain Reaction: ( NEET 2026)

Which of the following statements are not true regarding restriction endonucleases? A. They are called molecular scissors. B. These are the enzymes responsible for restricting the growth of bacteriophages in E. coli. C. They cut the DNA only at the centre of the palindromic sites. D. They remove nucleotides only from the ends of DNA fragments. E. They recognise specific palindromic base-pair sequences. ( NEET 2026)

Which of the following statements are correct with respect to DNA separation, isolation and visualization? A. The cutting of DNA is done by molecular scissors. B. The DNA fragments separate according to their size in an agarose gel, upon electrophoresis. C. The separated DNA fragments can be seen without staining when exposed to UV light. D. The separated DNA fragments, when stained with ethidium bromide, can be seen in visible light. ( NEET 2026)

Insertion of a foreign DNA at BamHI site in an E.coli cloning vector pBR322 results in the loss of antibiotic resistance towards: ( NEET 2026)