Steps of Recombinant DNA Technology — NEET Biology
✅ Asked in NEET 2023Recombinant DNA technology involves several steps in specific sequence such as isolation of DNA, fragmentation of DNA by restriction endonucleases, isolation of a desired DNA fragment, ligation of the DNA fragment into a vector, transferring the recombinant DNA into the host, culturing the host cells in a medium at large scale and extraction of the desired product. Let us examine each of these steps in some details.
NTA tests students' knowledge of the correct sequential order of recombinant DNA technology steps: isolation, fragmentation, desired fragment isolation, ligation into vector, host transfer, culturing, and product extraction. The most common student mistake is mixing up the order—for example, attempting ligation before fragmentation or transferring DNA before ligation. Students must memorize this exact sequence because skipping or reversing any step makes the entire process fail. To score correctly, remember the acronym IF-DFL-HCE (Isolation, Fragmentation, Desired-fragment, Ligation, Host-transfer, Culturing, Extraction) and visualize each step logically: you must break DNA first, select the right piece, then join it to a vector before introducing it to host cells.
Main steps in the formation of Recombinant DNA are given below. Arrange these steps in a correct sequence. (NEET 2023) A. Insertion of recombinant DNA into the host cell B. Cutting of DNA at specific location by restriction enzyme C. Isolation of desired DNA fragment D. Amplification of gene of interest using PCR
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