Palindromic DNA Sequences — NEET Biology
✅ Asked in NEET 2021For example, the following sequences reads the same on the two strands in 5' → 3' direction. This is also true if read in the 3' → 5' direction.
Palindromic sequences are DNA regions that read identically in the 5'→3' direction on both strands, making them symmetric and 'mirror-like'. NTA tests this because restriction enzymes recognize these palindromic sites, which is fundamental to biotechnology applications like recombinant DNA technology. Students commonly confuse palindromic sequences with complementary base pairing or forget that the reading direction matters on both strands. Remember: a true palindromic sequence reads the same when read 5'→3' on the top strand AND 5'→3' on the bottom strand—this is why restriction enzymes (which cut palindromic sites) are so predictable and useful in genetic engineering.
A specific recognition sequence identified by endonucleases to make cuts at specific positions within the DNA is: (NEET 2021)
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