Class 12 · Human Reproduction

Sperm-Ovum Fertilisation Process — NEET Biology

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📖 NCERT Source

The process of fusion of a sperm with an ovum is called fertilisation. During fertilisation, a sperm comes in contact with the zona pellucida layer of the ovum and induces changes in the membrane that block the entry of additional sperms. Thus, it ensures that only one sperm can fertilise an ovum. The secretions of the acrosome help the sperm enter into the cytoplasm of the ovum through the zona pellucida and the plasma

🖼️Related NCERT figure: Diagram showing an ovum surrounded by few sperms. The ovum is shown as a large circular structure with labeled parts including the zona pellucida, cells of the corona radiata, ovum center, and perivitelline space. Several sperm cells are shown around the ovum attempting fertilization. (Figure 2.10 Ovum surrounded by few sperms)
NCERT Biology · Class 12 · Chapter 2 · Paragraph 27
How NTA Uses This Concept

NTA tests whether students understand that fertilisation begins when a sperm contacts the zona pellucida (outer glycoprotein layer) of the ovum, which triggers the block to polyspermy—preventing entry of additional sperms. This ensures monogamy of fertilisation. Students often confuse whether the block occurs before or after sperm entry, or mix up which structure triggers the block. Key trap: thinking multiple sperms can fertilise one ovum, or that the block happens at the plasma membrane first. Remember: zona pellucida contact → membrane changes → polyspermy block → only one sperm enters. This concept is fundamental to understanding early embryonic development and is tested to assess reproductive biology clarity.

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Receptors for sperm binding in mammals are present on:

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