Class 12 · Molecular Basis of Inheritance

Radioactive Isotopes Label DNA & Protein — NEET Biology

✅ Asked in NEET 2023
📖 NCERT Source

They grew some viruses on a medium that contained radioactive phosphorus and some others on medium that contained radioactive sulfur. Viruses grown in the presence of radioactive phosphorus contained radioactive DNA but not radioactive protein because DNA contains phosphorus but protein does not. Similarly, viruses grown on radioactive sulfur contained radioactive protein but not radioactive DNA because DNA does not contain sulfur.

NCERT Biology · Class 12 · Chapter 5 · Paragraph 36
How NTA Uses This Concept

NTA tests whether students understand that phosphorus (P-32) is found only in DNA, not in proteins, while sulfur (S-35) is found only in proteins, not in DNA. This was the basis of the famous Hershey-Chase experiment proving DNA is genetic material. Students often confuse which isotope labels which molecule or forget that this principle depends on the chemical composition of each macromolecule. To get it right: memorize that DNA contains phosphorus (in phosphate groups) but no sulfur, while proteins contain sulfur (in amino acids methionine and cysteine) but no phosphorus. Use this distinction to predict labeling patterns in similar experiments.

Solve This NEET Question
QuestionNEET 2023

With reference to Hershey and Chase experiments, select the correct statements: (NEET 2023) A: Viruses grown in the presence of radioactive phosphorus contained radioactive DNA. B: Viruses grown on radioactive sulphur contained radioactive proteins. C: Viruses grown on radioactive phosphorus contained radioactive protein. D: Viruses grown on radioactive sulphur contained radioactive DNA. E: Viruses grown on radioactive protein contained radioactive DNA. Choose the correct option:

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