Biotechnology and its application — NEET Biology Questions 2026

Practice 323 NCERT-mapped questions from Class 12, Chapter 10 (Biotechnology). ~3% weightage in NEET. Includes MCQ, Assertion-Reason, and NEET 2025 long-form questions.

323 Questions Class 12 ~3% Weightage 4 Topics
230
MCQ Questions
40
Assertion-Reason
53
NEET 2025 Pattern
38
Previous Year Q

NCERT Mapping

Biotechnology and its application is from NCERT Class 12 Biology, Chapter 10, under the unit Biotechnology. This chapter carries approximately 3% weightage in NEET, based on analysis of the last 10 years of question papers. MedicNEET covers this chapter with 323 questions — every question built from line-by-line NCERT analysis.

Topics in Biotechnology and its application

Sample Questions — Biotechnology and its application

Try 5 free questions below. Get all 323 in the MedicNEET app.

MCQ
Which set correctly contrasts two biotech strategies and their mechanisms?
  • a) ELISA–antibody production in plants; RNAi–antigen binding
  • b) PCR–nucleic acid amplification; Bt–alkaline-activated protoxin
  • c) Gene therapy–protein digestion; Transgenics–natural selection
  • d) Golden rice–pesticide detox; ADA therapy–DNA vaccination
✅ Correct Answer: B
PCR amplifies nucleic acids; Bt protoxin activation requires alkaline insect midgut.
MCQ
Q10. Which of the following are used to transfer genes in crop biotechnology? 1. E. coli 2. Agrobacterium tumefaciens 3. Retrovirus 4. Plasmids
  • A. 1, 2 and 3 only
  • B. 2 and 4 only
  • C. 1 and 4 only
  • D. 2, 3, and 4 only
✅ Correct Answer: B
Agrobacterium and plasmids are gene transfer agents in plants; retrovirus is used in animal systems.
👆 Want all 323 Biotechnology and its application questions? Get them in the app. Download MedicNEET →
MCQ
Why are transgenic animals used to test vaccine safety instead of directly on humans?
  • A. They generate more antibodies
  • B. They are cheaper than humans
  • C. They produce viral proteins
  • D. They mimic human immune responses
✅ Correct Answer: D
Transgenic mice help predict vaccine safety outcomes before proceeding to human trials.
Assertion-Reason
Assertion (A): Current treatments for ADA deficiency provide therapeutic benefits. Reason (R): Both bone marrow transplantation and enzyme replacement therapy are not completely curative approaches.
  • A. If both assertion and reason are true and the reason is the correct explanation of the assertion.
  • B. If both assertion and reason are true but reason is not the correct explanation of the assertion.
  • C. If assertion is true but reason is false.
  • D. If the assertion and reason both are false.
✅ Correct Answer: B
Both statements are true from NCERT. NCERT states treatments exist: “In some children ADA deficiency can be cured by bone marrow transplantation; in others it can be treated by enzyme replacement therapy” and “The problem with both of these approaches that they are not completely curative.” However, the Reason doesn’t explain WHY treatments provide benefits - it states their limitation. Both facts are true but unrelated explanatorily.
NEET 2025 Pattern
Which of the following statements correctly describe the roles of transgenic animals? S1: They are designed to study how genes are regulated and affect normal body functions and development. S2: They are used to produce biological products such as human α-1-antitrypsin for treating specific human diseases. S3: Transgenic animals are primarily created to enhance the overall biomass and yield of agricultural livestock. S4: Transgenic mice are utilized for testing the safety of vaccines before human application. S5: They serve as models for human diseases like cancer, cystic fibrosis, and Alzheimer’s, aiding in new treatment investigations.
  • A. S1, S2, S3 and S4
  • B. S2, S3, S4 and S5
  • C. S1, S2, S4 and S5
  • D. All of the above
✅ Correct Answer: C
Transgenic animals are designed to study gene regulation and its effects on normal body functions and development (S1 is correct). They are used to produce biological products like human α-1-antitrypsin for therapeutic purposes (S2 is correct). Enhancing overall biomass and yield of agricultural livestock is not listed as a primary role of transgenic animals in the NCERT text (S3 is incorrect). Transgenic mice are indeed used for vaccine safety testing (S4 is correct). They serve as models for various human diseases, including cancer, cystic fibrosis, and Alzheimer’s, to investigate new treatments (S5 is correct). Therefore, statements S1, S2, S4, and S5 are correct. Tested Concept: Multiple roles and applications of transgenic animals.

Practice All 323 Questions from Biotechnology and its application

You've seen 5 sample questions. Get all 323 with detailed NCERT-mapped explanations in the MedicNEET app.

📲 Download MedicNEET Free
Available on Android • 12,771 Biology Questions • 3 Formats

Frequently Asked Questions

How many Biotechnology and its application questions does MedicNEET have?
MedicNEET has 323 questions for Biotechnology and its application, including 230 MCQs, 40 Assertion-Reason questions, and 53 NEET 2025-style long-form questions.
What is the NEET weightage of Biotechnology and its application?
Biotechnology and its application carries approximately 3% weightage in NEET, based on analysis of the last 10 years of papers. It falls under the Biotechnology unit of Class 12 Biology.
Are Biotechnology and its application questions NCERT-based?
Yes, all MedicNEET Biotechnology and its application questions are built from line-by-line analysis of NCERT Class 12 textbook. Every question maps to specific NCERT pages and concepts.
📲 Download MedicNEET — 12,771 Biology Questions