Epidermis is usually single-layered. Epidermal cells are parenchymatous with a small amount of cytoplasm lining the cell wall and a large vacuole. The outside of the epidermis is often covered with a waxy thick layer called the cuticle which prevents the loss of water. Cuticle is absent in roots. Stomata are structures present in the epidermis of leaves. Stomata regulate the process of transpiration and gaseous exchange. Each stoma is composed of two bean-shaped cells known as guard cells which enclose stomatal pore. In grasses, the guard cells are dumb-bell shaped. The outer walls of guard cells (away from the stomatal pore) are thin and the inner walls (towards the stomatal pore) are highly thickened. The guard cells possess chloroplasts and regulate the opening and closing of stomata. Sometimes, a few epidermal cells, in the vicinity of the guard cells become specialised in their shape and size and are known as subsidiary cells. The stomatal aperture, guard cells and the surrounding subsidiary cells are together called stomatal apparatus.
Guard cells are the primary regulators of stomatal opening and closing in plant leaves. NTA frequently tests their structural differences between dicots and grasses—specifically that grass guard cells are dumb-bell shaped while dicot guard cells are bean-shaped, with inner walls toward the pore being highly thickened. Students commonly confuse these shapes or forget that guard cells contain chloroplasts, which is crucial for their function. Remember: guard cells are always present in pairs, enclose the stomatal pore, and work with subsidiary cells to form the complete stomatal apparatus. The thickened inner wall design allows guard cells to expand/contract and regulate transpiration and gas exchange efficiently.
Stomata in grass leaf are: (NEET 2018)
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