Enzyme Activation Energy Reduction — NEET Biology

Which of the above statements are correct? S1: Enzymes reduce the difference in potential energy between the stable substrate and the transition state. S2: The catalytic effect of an enzyme is depicted as a downward shift in the energy level of the product (P) relative to the substrate (S) in the energy diagram. S3: Enzymes facilitate the transformation of substrate to product by stabilizing intermediate structural states, making them less unstable. S4: Enzymes do not alter the overall free energy change (ΔG) of a reaction, only the rate at which equilibrium is reached. S5: The increased reaction rate achieved by enzymes is solely due to the prolonged binding of the substrate at the active site.