The binding of the substrate induces the enzyme to alter its shape, fitting more tightly around the substrate.
Which of the above statements are correct? S1: The binding of the substrate to the active site induces a conformational change in the enzyme, leading to a tighter fit. S2: The enzyme-product complex (EP) is a short-lived intermediate formed before the release of the product. S3: Enzymes are consumed during the conversion of substrate into product and must be regenerated for subsequent reactions. S4: The active site of an enzyme is always a deep crevice or pocket where the substrate fits perfectly without any induced structural changes. S5: The formation of the enzyme-substrate (ES) complex is an optional step in enzyme catalysis, dependent on specific reaction conditions.
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