Hydrolases and Bond Hydrolysis — NEET Biology
✅ Asked in NEET 2024Match List I with List IIList I A. Lipase B. Nuclease C. Protease D. Amylase List II I. Peptide bond II. Ester bond III. Glycosidic bond IV. Phosphodiester bond Choose the correct option:
Match List I with List IIList I A. Lipase B. Nuclease C. Protease D. Amylase List II I. Peptide bond II. Ester bond III. Glycosidic bond IV. Phosphodiester bond Choose the correct option:
Answer & NCERT explanation
Correct answer: C — A–II, B–IV, C–I, D–III
Lipase breaks ester bonds in lipids (A-II), Nuclease breaks phosphodiester bonds in nucleic acids (B-IV), Protease breaks peptide bonds in proteins (C-I), and Amylase breaks glycosidic bonds in starch (D-III). This matches the substrate-bond specificity of each enzyme according to NCERT.
Read more NCERT concept on the PYQ
Hydrolases: Enzymes catalysing hydrolysis of ester, ether, peptide, glycosidic, C-C, C-halide or P-N bonds.
Hydrolases are enzymes that break down various types of chemical bonds by adding water molecules (hydrolysis). NTA tests this concept because hydrolases are crucial in digestion and cellular metabolism, breaking down biomolecules like proteins, carbohydrates, and fats. Students commonly mistake hydrolases for enzymes that form bonds instead of breaking them—remember they always ADD water to cleave bonds. The key is recognizing different hydrolases by the bonds they target: peptidases break peptide bonds, lipases break ester bonds in fats, amylases break glycosidic bonds in carbohydrates. Knowing these specifics helps you match enzyme types to their substrates and predict digestion pathways correctly.
What does NCERT say about Hydrolases Enzymes catalysing hydrolysis?
Has this concept appeared in NEET?
Which chapter is this from?
Through deep analysis of NEET and NTA, 88 of 90 questions from the NEET 2026 paper were matched straight from the MedicNEET Biology question bank.
88/90MedicNEET's Biology question bank is built from the same NCERT lines NTA picks repeatedly. Not random MCQs — questions crafted exactly like NTA crafts them.