Role of Cofactors in Enzyme Activity — NEET Biology

Which of the above statements are NOT correct? S1: The apoenzyme is the protein portion of an enzyme, becoming catalytically active only after binding with a non-protein cofactor. S2: Prosthetic groups, like haem in catalase, are organic compounds characterized by their tight and usually transient association with the apoenzyme. S3: Co-enzymes are organic compounds whose essential chemical components are often derived from vitamins, and they typically associate with the apoenzyme only transiently during catalysis. S4: Metal ions function as cofactors by forming coordination bonds exclusively with the enzyme's active site, without any direct interaction with the substrate. S5: The removal of any type of cofactor from its enzyme invariably results in a complete and irreversible loss of catalytic activity.