Class 12 · Molecular Basis of Inheritance

Hershey-Chase Experiment — NEET Biology

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Radioactive phages were allowed to attach to E. coli bacteria. Then, as the infection proceeded, the viral coats were removed from the bacteria by agitating them in a blender. The virus particles were separated from the bacteria by spinning them in a centrifuge.

NCERT Biology · Class 12 · Chapter 5 · Paragraph 37
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Arrange the following key steps of Hershey and Chase’s experiment in the correct chronological sequence: 1. Radioactive phages were allowed to infect E. coli bacteria. 2. Some viruses were grown in medium containing radioactive sulfur (³⁵S) → labeled proteins. 3. Some viruses were grown in medium containing radioactive phosphorus (³²P) → labeled DNA. 4. After infection, phage coats were removed by blending and separated by centrifugation. 5. Bacteria infected with ³²P-labeled phages became radioactive, showing DNA entered cells.

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