Now that you know that the C₄ plants lack photorespiration, you probably can understand why productivity and yields are better in these plants. In addition, these plants show tolerance to higher temperatures.
Match the following characteristics of C₄ plants with their associated mechanisms and physiological advantages: Column I (C₄ Characteristic) A. Kranz Anatomy B. PEPcase activity C. CO₂ transport to bundle sheath D. RuBisCO location in bundle sheath Column II (Mechanism/Enzyme) I. High CO₂ affinity II. Thick-walled bundle sheath cells III. C₄ acids decarboxylation in bundle sheath DIV. Spatial separation of reactions Column III (Physiological Advantage) 1. Increased CO₂ fixation at low atmospheric CO₂ 2. Minimised oxygenase activity of RuBisCO 3. Tolerance to higher temperatures and light intensities 4. Greater productivity and biomass production Which of the following options provides the correct set of matches?
Correct answer: A — A-II-3, B-I-1, C-III-2, D-IV-4
The core concept tested is the advantages of the C₄ pathway and the underlying mechanisms. A. Kranz Anatomy is characterized by large, thick-walled bundle sheath cells (II) that lack intercellular spaces. This anatomy allows for the spatial separation of initial CO₂ fixation and the Calvin cycle, contributing to the greater productivity and biomass production (4) by efficiently concentrating CO₂. B. PEPcase activity involves the primary fixation of CO₂ by PEPcase, an enzyme with high CO₂ affinity (I) that does not bind O₂. This allows for increased CO₂ fixation even at low atmospheric CO₂ concentrations (1). C. CO₂ transport to bundle sheath involves the C₄ acids (malic/aspartic acid) being transported to bundle sheath cells where they undergo decarboxylation (III) to release CO₂. This CO₂ concentrating mechanism ensures that RuBisCO operates in a high CO₂ environment, minimizing its oxygenase activity (2) and thus photorespiration. D. RuBisCO location in bundle sheath cells, coupled with the efficient CO₂ concentrating mechanism (IV, representing spatial separation and CO2 pump), ensures that C₄ plants can tolerate higher temperatures and light intensities (3) by effectively suppressing photorespiration. Matching the columns: - A (Kranz Anatomy) matches with II (Thick-walled bundle sheath cells) and 4 (Greater productivity and biomass production). - B (PEPcase activity) matches with I (High CO₂ affinity) and 1 (Increased CO₂ fixation at low atmospheric CO₂). - C (CO₂ transport to bundle sheath) matches with III (C₄ acids decarboxylation in bundle sheath) and 2 (Minimised oxygenase activity of RuBisCO). - D (RuBisCO location in bundle sheath) matches with IV (Spatial separation of reactions) and 3 (Tolerance to higher temperatures and light intensities). Therefore, the correct option is A-II-4, B-I-1, C-III-2, D-IV-3. This question evaluates the student's understanding of how specific anatomical and biochemical features of C₄ plants contribute to their ecological and physiological advantages.
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