Class 11 · Photosynthesis in Higher Plants

CO₂ Requirement in Photosynthesis — NEET Biology

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Another experiment you may have carried out where a part of a leaf is enclosed in a test tube containing some KOH soaked cotton (which absorbs CO₂), while the other half is exposed to air. The setup is then placed in light for some time. On testing for the presence of starch later in the two parts of the leaf, you must have found that the exposed part of the leaf tested positive for starch while the portion that was in the tube, tested negative. This showed that CO₂ was required for photosynthesis. Can you explain how this conclusion could be drawn?

🖼️Related NCERT figure: Priestley's experiment shown in four stages (a, b, c, d). Shows bell jars with candles, mice, and plants demonstrating the relationship between plant photosynthesis and air purification. (Figure 11.1 Priestley's experiment)
NCERT Biology · Class 11 · Chapter 11 · Paragraph 8
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How did the use of KOH in a test tube prove the necessity of CO₂ in photosynthesis?

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