• There is a sequential, orderly pathway functioning, with one substrate forming the next and with glycolysis, TCA cycle and ETS pathway following one after another. • The NADH synthesised in glycolysis is transferred into the mitochondria and undergoes oxidative phosphorylation. • None of the intermediates in the pathway are utilised to synthesise any other compound. • Only glucose is being respired – no other alternative substrates are entering in the pathway at any of the intermediary stages.
Which of the following statements are correct concerning the assumptions made while calculating the net gain of ATP in the respiratory balance sheet? S1. Glycolysis, TCA cycle, and ETS are considered to function in an orderly, sequential manner. S2. All NADH molecules synthesized during glycolysis are assumed to be efficiently transferred into the mitochondrial matrix. S3. Intermediates of the pathway are assumed to be readily utilized for the biosynthesis of other compounds whenever required. S4. Only glucose is considered as the primary respiratory substrate, and no other substrates enter the pathway at intermediary stages. S5. Enzymatic rates are assumed to be constantly regulated by various cellular mechanisms to maintain a steady ATP yield.
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