Fungal Sexual Reproduction: Plasmogamy, Karyogamy and Meiosis

Biology · Biological Classification · NEET

Fungi reproduce sexually in three fixed steps. First plasmogamy joins the protoplasm (cytoplasm) of two cells. Then karyogamy fuses their two nuclei to make a diploid (2n) nucleus. Finally meiosis (reduction division) makes haploid spores. Memory hook: "P-K-M = Protoplasm, then Kernels (nuclei), then Make spores."
Sexual Cycle in Fungi: 3 StepsTwo cells (n) + (n)Plasmogamycytoplasm fusesn + n (nuclei apart)Karyogamynuclei fuseDiploid (2n)MeiosisreductionHaploid spores (n)
The fixed 3-step fungal sexual cycle: plasmogamy fuses cytoplasm (n+n), karyogamy fuses nuclei into a diploid (2n) cell, then meiosis makes haploid (n) spores.

Your doubts, answered

What is the difference between plasmogamy and karyogamy?

Plasmogamy means fusion of the protoplasm (cytoplasm) of two gametes or cells. Their two nuclei come close but do NOT fuse yet. Karyogamy is the next step, where those two nuclei fuse into one diploid (2n) nucleus. Simple trick: 'plasm' = plasma/cytoplasm fuses first; 'karyo' = karyon = nucleus fuses second.

What is the correct order of the three steps?

The order is fixed: (1) Plasmogamy, (2) Karyogamy, (3) Meiosis. Cytoplasm fuses, then nuclei fuse to make 2n, then meiosis reduces it back to haploid (n) spores. NEET loves this exact sequence in match-the-column questions, so never swap the order.

Does karyogamy make a diploid or haploid cell?

Karyogamy makes a DIPLOID (2n) nucleus, because it fuses two haploid (n) nuclei into one. This is the only diploid stage in most fungi. Meiosis then follows and brings it back to haploid (n) spores. So: karyogamy = builds up to 2n, meiosis = cuts down to n.

Why does meiosis come last and not first?

Meiosis must reduce the diploid nucleus (formed by karyogamy) back to haploid. So a diploid nucleus must exist first. That is why the order is plasmogamy, then karyogamy (makes 2n), then meiosis (makes n spores). Meiosis is called reduction division because it halves the chromosome number.

Where does the dikaryon (n+n) stage fit in?

In some fungi (ascomycetes and basidiomycetes), after plasmogamy the two nuclei do NOT fuse right away. The cell holds two separate nuclei (n+n) — this is the dikaryon or dikaryophase. Karyogamy is delayed until later, then meiosis follows. In lower fungi (like phycomycetes), karyogamy happens immediately, so there is no dikaryon.

Are the spores made after meiosis haploid or diploid?

The spores made after meiosis are HAPLOID (n). Meiosis takes the diploid (2n) nucleus and halves it. Examples of these sexual spores are oospores, ascospores and basidiospores. These form inside distinct structures called fruiting bodies.

⚠️ The NEET trap
Plasmogamy is the fusion of two nuclei.
Plasmogamy is the fusion of protoplasm (cytoplasm) of two gametes. It is KARYOGAMY that fuses the two nuclei.
🧠 Match-the-column PYQs swap these two. Remember: Plasmo = plasma/protoplasm; Karyo = karyon/nucleus. Protoplasm always fuses BEFORE the nuclei.

Real NEET questions

ReNEET 2026

Match List-I with List-II: List-I A. Fusion of protoplasms between gametes B. Fusion of two nuclei C. Generation of haploid spores List-II I. Meiosis II. Plasmogamy III. Karyogamy Choose the correct answer:

A · A-II, B-III, C-I
B · A-II, B-I, C-III
C · A-III, B-II, C-I
D · A-I, B-III, C-II
Solution: Plasmogamy is fusion of protoplasms of two gametes, so A-II. Karyogamy is fusion of the two nuclei, so B-III. Meiosis generates haploid spores, so C-I. Correct match: A-II, B-III, C-I.
NEET 2018

After karyogamy followed by meiosis, spores are produced exogenously in:

A · Agaricus
B · Alternaria
C · Neurospora
D · Saccharomyces
Solution: Agaricus is a basidiomycete. Karyogamy and meiosis occur in the basidium, producing four basidiospores that are borne EXOGENOUSLY (on the outside) on the basidium. Neurospora and Saccharomyces are ascomycetes with endogenous ascospores; Alternaria is a deuteromycete with asexual conidia. So the answer is Agaricus.

Solved Biological Classification NEET PYQs

Try the real previous-year questions from this chapter — each with the answer and a full solution.

See all 36 Biological Classification NEET PYQs ›
Next concept: Dikaryon and Dikaryophase (n+n) in Fungi ExplainedKeep learning — 2 minFeeling ready? Solve the Biological Classification NEET PYQs ›Or practice on your phone — get the free MedicNEET app ›

Frequently asked

What are the three steps of sexual reproduction in fungi?

Plasmogamy (fusion of protoplasm), karyogamy (fusion of two nuclei to form a diploid nucleus), and meiosis (reduction division that forms haploid spores). This exact order is a common NEET question.

What is plasmogamy in simple words?

Plasmogamy is when the cytoplasm (protoplasm) of two fungal cells or gametes fuses together. The two nuclei come inside one cell but stay separate for now.

What is karyogamy?

Karyogamy is the fusion of the two nuclei to make a single diploid (2n) nucleus. It comes right after plasmogamy in fungi that fuse nuclei immediately, or after a delay in ascomycetes and basidiomycetes.

Why is meiosis needed in fungal sexual reproduction?

Karyogamy makes a diploid (2n) nucleus. Meiosis (reduction division) halves it back to haploid (n), producing haploid sexual spores like oospores, ascospores or basidiospores. This keeps the chromosome number stable across generations.

What is the difference between endogenous and exogenous spores here?

Ascospores form INSIDE a sac-like ascus (endogenous). Basidiospores form OUTSIDE, on the surface of the basidium (exogenous). NEET 2018 tested that exogenous spores after karyogamy and meiosis point to Agaricus (a basidiomycete).