Ribosome Structure: 70S vs 80S and Their Subunits

Biology · Cell: The Unit of Life · NEET

A 70S ribosome is found in prokaryotes and is made of two subunits: a larger 50S and a smaller 30S. An 80S ribosome is found in eukaryotes (in the cytoplasm) and is made of a larger 60S and a smaller 40S. Memory hook: "big + small do not add up because S is a floating (sedimentation) speed, not a weight" — so 50 + 30 is NOT 80, it is 70.
Ribosome Subunits: 70S vs 80SPROKARYOTIC 70S50S (large)30S (small)50S + 30S = 70Sbacteria, mitochondria, chloroplastEUKARYOTIC 80S60S (large)40S (small)60S + 40S = 80Splant & animal cytoplasmS = Svedberg (sedimentation), not mass — values do not add up
Each ribosome has one large and one small subunit. Prokaryotic 70S = 50S + 30S; eukaryotic 80S = 60S + 40S. The S value is sedimentation speed, so the subunit numbers do not add up arithmetically.

Your doubts, answered

Why does 50S + 30S make 70S and not 80S? The numbers do not add up!

This is the number-one doubt and NTA loves it. The 'S' stands for Svedberg unit, which measures how fast a particle settles (sediments) in a centrifuge, NOT its mass. Sedimentation speed depends on both size AND shape, so the values are NOT simply added. When the 50S and 30S subunits join, the combined shape sediments at 70S (not 80S). Same logic for eukaryotes: 60S + 40S = 80S, not 100S. Just memorise the final pairs; never add the subunit numbers.

Which organism has 70S ribosomes and which has 80S?

70S ribosomes are found in ALL prokaryotes (bacteria, cyanobacteria, mycoplasma). 80S ribosomes are found in the cytoplasm of eukaryotes (plant, animal, fungi cells). The important NEET twist: eukaryotes ALSO contain 70S ribosomes inside their mitochondria and chloroplasts. So a plant cell has both 80S (cytoplasm) and 70S (in mitochondria + chloroplast).

What are the subunits of 70S and 80S ribosomes? (exact NEET values)

70S ribosome (prokaryotic) = 50S (larger subunit) + 30S (smaller subunit). 80S ribosome (eukaryotic) = 60S (larger subunit) + 40S (smaller subunit). NEET 2025 asked this exact fact. Wrong distractor values you must reject: 60S+20S, 60S+30S, 50S+20S — all fake. Only 60S+40S and 50S+30S are correct.

Are ribosomes membrane-bound? Do they have DNA?

No. Ribosomes are NON-membrane-bound organelles — they have no membrane at all. They are made of ribosomal RNA (rRNA) and proteins only; they do NOT contain DNA. NCERT says they are 'not surrounded by any membrane'. This makes them the only organelle present in both prokaryotes and eukaryotes (NEET 2026 tested this).

What is a ribosome made of and what does it do?

A ribosome is a ribonucleoprotein — made of rRNA plus proteins (the eukaryotic 80S ribosome has about 80 different proteins; NEET 2023 asked this). Its job is protein synthesis (translation). In prokaryotes ribosomes lie free in the cytoplasm or attach to the plasma membrane; in eukaryotes they lie free OR attach to the endoplasmic reticulum to form rough ER (RER).

70S vs 80S — which is bigger?

80S is the bigger, heavier ribosome (eukaryotic). 70S is smaller (prokaryotic). Higher S value = settles faster = generally larger/heavier here. But remember S is sedimentation, so use this only as a rough guide, not for adding subunits.

⚠️ The NEET trap
The two subunits of an 80S ribosome are 60S and 20S, and of a 70S ribosome are 50S and 20S.
80S = 60S + 40S; 70S = 50S + 30S. The smaller subunits are 40S and 30S, never 20S.
🧠 NTA plants fake '20S' subunit values (NEET 2025 statements D and E). Lock in: eukaryote 60/40, prokaryote 50/30. If you ever see a '20S' subunit, it is a trap.

Real NEET questions

NEET 2025

From the statements given below choose the correct option: A. The eukaryotic ribosomes are 80S and prokaryotic ribosomes are 70S. B. Each ribosome has two sub-units. C. The two sub-units of 80S ribosome are 60S and 40S while that of 70S are 50S and 30S. D. The two sub-units of 80S ribosome are 60S and 20S and that of 70S are 50S and 20S. E. The two sub-units of 80S are 60S and 30S and that of 70S are 50S and 30S.

A · A, B, E are true
B · B, D, E are true
C · A, B, C are true
D · A, B, D are true
Solution: A is true (eukaryotes 80S, prokaryotes 70S). B is true (each ribosome has two subunits). C is true (80S = 60S + 40S; 70S = 50S + 30S). D and E give wrong values (20S and 30S smaller subunits are fake). So only A, B, C are correct — option (C). NCERT Ch 8: 'The two subunits of 80S ribosomes are 60S and 40S while that of 70S ribosomes are 50S and 30S.'
NEET 2023 Phase 1

How many different proteins does the ribosome consist of?

A · 80
B · 60
C · 40
D · 20
Solution: A ribosome is a ribonucleoprotein made of rRNA and proteins. The eukaryotic 80S ribosome is built from roughly 80 different protein molecules across its 60S and 40S subunits, so the answer is 80. This is an extension of NCERT, which states ribosomes are composed of RNA and proteins and the 80S ribosome has 60S and 40S subunits.
NEET 2019 Odisha

Which of the following nucleic acids is present in an organism having 70S ribosomes only?

A · Single stranded DNA with protein coat
B · Double stranded circular naked DNA
C · Double stranded DNA enclosed in nuclear membrane
D · Double stranded circular DNA with histone proteins
Solution: An organism with ONLY 70S ribosomes is a prokaryote. Its genetic material is naked, double-stranded, circular DNA — not enclosed by a nuclear membrane and without histones. Options with nuclear membrane or histones describe eukaryotes (80S); single-stranded DNA with a protein coat describes a virus. So the answer is double stranded circular naked DNA.

Solved Cell: The Unit of Life NEET PYQs

Try the real previous-year questions from this chapter — each with the answer and a full solution.

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Frequently asked

What does the S in 70S and 80S stand for?

S stands for Svedberg unit. It measures the sedimentation rate — how fast a particle settles when spun in an ultracentrifuge. It depends on size and shape, not just mass, which is why subunit values do not add up simply.

Do prokaryotes and eukaryotes both have ribosomes?

Yes. Ribosomes are the only organelle present in both. Prokaryotes have 70S ribosomes. Eukaryotes have 80S in their cytoplasm, plus 70S inside their mitochondria and chloroplasts.

Are ribosomes membrane-bound organelles?

No. Ribosomes have no membrane. They are non-membrane-bound organelles made of rRNA and protein, and they contain no DNA.

What is the size of a prokaryotic ribosome?

NCERT states prokaryotic ribosomes are about 15 nm by 20 nm and are associated with the plasma membrane of the cell.

Why do mitochondria and chloroplasts have 70S ribosomes?

Because they are semi-autonomous organelles believed to have evolved from ancient prokaryotes (endosymbiosis). They keep their own circular DNA and 70S ribosomes, just like bacteria — a favourite NEET point.