Mendel's Law of Segregation (Law of Purity of Gametes) Explained

Biology · Principles of Inheritance and Variation · NEET

Every organism has two copies (alleles) of each gene. Mendel's Law of Segregation says these two alleles separate (segregate) from each other when gametes are made, so each gamete gets only ONE allele. This is why it is also called the "Law of Purity of Gametes" — a gamete is never mixed, it is pure for one allele. Memory hook: "Two in, one out" — two alleles in the parent cell, only one goes out into each gamete.
Law of Segregation: Alleles separate during gamete formationParent cellT ttwo allelesmeiosisTtpure gametes (one allele each)Fertilisation combinesone allele from each parentTT : Tt : tt = 1 : 2 : 13 tall : 1 dwarf (phenotype)Each gamete stays PURE for one allele = "Law of Purity of Gametes"
The two alleles (T and t) of a Tt parent separate during meiosis, so each gamete carries only one pure allele. Random fertilisation then gives the classic 1:2:1 genotype (3:1 phenotype) ratio in F2.

Your doubts, answered

What is the Law of Segregation in simple words?

A parent has two alleles for a gene (for example T and t). When the parent makes gametes (eggs or pollen), these two alleles separate. One gamete gets T, another gamete gets t. No gamete gets both. So half the gametes carry one allele and half carry the other. This separation is what we call segregation.

Why is it also called the Law of Purity of Gametes?

Because each gamete stays PURE for one allele — it carries only one, never a mixture of both. Even in a Tt parent, the T and t do not blend inside a gamete. A gamete is either T or t, clean and pure. That is why the two names mean the same law.

At what stage do the two alleles actually separate?

They separate during MEIOSIS, when gametes are formed. The two alleles of a gene sit on two homologous chromosomes. When these homologous chromosomes separate (in Anaphase I of meiosis), the alleles they carry go to different cells. So meiosis is the physical reason behind segregation. This link was later explained by the Chromosomal Theory of Inheritance.

How is Law of Segregation different from Law of Dominance?

Law of Dominance explains what you SEE — in a Tt plant, only the dominant trait (tall) shows in F1. Law of Segregation explains what happens INSIDE during gamete formation — the alleles separate so the hidden recessive can reappear in F2. Dominance explains the 3:1 look; segregation explains WHY the recessive comes back and gives the 1 in that 3:1.

Does the Law of Segregation apply to incomplete dominance (like the pink Snapdragon)?

Yes. This is a common NEET trap. Even in incomplete dominance, the alleles still separate cleanly during gamete formation. In Snapdragon, red (RR) x white (rr) gives pink (Rr) F1, and selfing gives 1 Red : 2 Pink : 1 White in F2. The red and white parents REAPPEAR in F2 — that only happens because the alleles were pure and segregated. So the Law of Segregation ALWAYS applies; only the Law of Dominance changes.

How does segregation give the 3:1 ratio in F2?

In a Tt x Tt cross, each parent's alleles segregate, so gametes are 1/2 T and 1/2 t. When they combine randomly: TT, Tt, Tt, tt. Genotype ratio is 1:2:1. Because T is dominant, the phenotype is 3 tall : 1 dwarf. The recessive dwarf reappears only because the alleles stayed pure and separated.

⚠️ The NEET trap
In incomplete dominance (pink Snapdragon), the Law of Segregation does not apply because F1 is a blended intermediate.
The Law of Segregation always applies. Even in incomplete dominance the alleles stay pure and separate, which is exactly why the original red and white parents reappear in the F2 (1 Red : 2 Pink : 1 White). Only the Law of Dominance is not followed here.
🧠 If parental types come back in F2, alleles were pure and DID segregate — blending in F1 does NOT break segregation.

Real NEET questions

NEET 2019

In Antirrhinum (Snapdragon), a red flower was crossed with a white flower and in F1 generation pink flowers were obtained. When pink flowers were selfed, the F2 generation showed white, red and pink flowers. Choose the incorrect statement from the following:

A · This experiment does not follow the Principle of Dominance.
B · Pink colour in F1 is due to incomplete dominance.
C · Ratio of F2 is 1/4 (Red) : 2/4 (Pink) : 1/4 (White)
D · Law of Segregation does not apply in this experiment
Solution: Snapdragon flower colour is incomplete dominance: red (RR) x white (rr) gives pink (Rr) F1, and F2 shows 1 Red : 2 Pink : 1 White. The Law of Dominance is NOT followed (F1 is intermediate pink). But the Law of Segregation STILL applies — the alleles stayed pure and separated, which is why red and white parents reappear in F2. So the statement 'Law of Segregation does not apply' is INCORRECT, making (D) the answer.

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Frequently asked

Who gave the Law of Segregation?

Gregor Johann Mendel proposed it from his monohybrid crosses on garden pea (Pisum sativum). It is called Mendel's Second Law of Inheritance.

Is the Law of Segregation the same as the Law of Purity of Gametes?

Yes. Both names describe the same idea: the two alleles separate during gamete formation, so each gamete carries only one pure allele.

Is the Law of Segregation universal?

Yes, it is considered a universal law with no known exception, because it depends on the separation of homologous chromosomes during meiosis, which happens in all sexually reproducing organisms.

What is the difference between segregation and independent assortment?

Segregation deals with ONE gene pair separating during gamete formation. Independent assortment (Mendel's Third Law) deals with TWO or more gene pairs separating independently of each other.