What is the Electron Transport System (ETS)?

Biology · Respiration in Plants · NEET

The Electron Transport System (ETS) is the last stage of aerobic respiration. It is a chain of carriers in the inner mitochondrial membrane that takes electrons from NADH and FADH2 and passes them step by step to oxygen, and this is where most ATP is made. Memory hook: think of ETS as a "down-hill staircase" where electrons fall step by step, and each step drops a little energy that the cell saves as ATP.
Electron Transport System (Inner Mitochondrial Membrane)Electrons flow down-hill (high to low energy)INADHIIFADH2IIIbc1IVcyt c oxVATPsynthaseubiquinonecyt cO2 + H+ → H2O (final acceptor)H+ flow → ATP
Electrons from NADH (Complex I) and FADH2 (Complex II) flow down the chain through ubiquinone, Complex III, cytochrome c, and Complex IV to oxygen (the final acceptor, forming water). Complex V (ATP synthase) then uses the proton flow to make ATP.

Your doubts, answered

Is the ETS the same as the Krebs cycle?

No. The Krebs cycle happens in the mitochondrial matrix and its job is to release CO2 and load electrons onto NADH and FADH2. The ETS is a separate, later stage. It happens in the inner mitochondrial membrane and its job is to unload those electrons onto oxygen and make ATP. Krebs cycle = fills the carriers; ETS = empties the carriers.

Where exactly does the electron transport system take place?

In plants and other cells with mitochondria, the ETS is located on the inner mitochondrial membrane (the folded cristae). This is different from glycolysis, which happens in the cytoplasm. Keep this straight for NEET: glycolysis = cytoplasm, Krebs cycle = matrix, ETS = inner membrane.

What actually carries the electrons into the ETS?

NADH and FADH2 are the carriers. They were made in glycolysis, the link reaction, and the Krebs cycle. NADH gives its electrons to Complex I (NADH dehydrogenase). FADH2 gives its electrons through Complex II (succinate dehydrogenase). From there the electrons move to ubiquinone, then cytochrome c, and finally to Complex IV.

Does the ETS make ATP directly?

No. The ETS itself does not make ATP. As electrons move through the complexes, protons (H+) are pushed across the inner membrane, building a proton gradient. ATP is then made by a separate enzyme, ATP synthase (Complex V), when protons flow back through it. So ETS builds the gradient, ATP synthase makes the ATP.

Why is oxygen needed in the ETS?

Oxygen is the final acceptor of electrons and hydrogen at the end of the chain. It combines with electrons and H+ to form water. Its role is only at this terminal stage, but it is vital: by removing hydrogen it keeps the whole chain moving. Without oxygen the electrons have nowhere to go, the chain stops, and aerobic ATP production halts.

How much ATP do NADH and FADH2 give in the ETS?

By the NCERT count, one NADH gives 3 ATP and one FADH2 gives 2 ATP. FADH2 gives less because it enters later (at Complex II), so it pumps fewer protons. Do not reverse these numbers; that swap is a very common NEET trap.

⚠️ The NEET trap
One NADH gives 2 ATP and one FADH2 gives 3 ATP.
One NADH gives 3 ATP and one FADH2 gives 2 ATP, because FADH2 enters the chain later at Complex II and pumps fewer protons.
🧠 NADH enters first at Complex I, so it 'wins more' (3 ATP). FADH2 joins late at Complex II, so it 'gets less' (2 ATP). First in = more ATP.

Real NEET questions

NEET 2019 · NEET 2021 · ReNEET 2026

Which of the following statements is incorrect?

A · ATP is synthesized through complex V
B · Oxidation-reduction reactions produce proton gradient in respiration
C · During aerobic respiration, role of oxygen is limited to the terminal stage
D · In ETC, one molecule of NADH + H+ gives rise to 2 ATP molecules, and one FADH2 gives rise to 3 ATP molecules
Solution: Option D is incorrect because the numbers are reversed. In the ETS, one NADH gives 3 ATP and one FADH2 gives 2 ATP. The other three statements are correct: ATP synthase is Complex V, oxidation-reduction reactions of the ETS build the proton gradient, and oxygen acts only at the terminal stage as the final electron and hydrogen acceptor.
NEET 2025

The complex II of mitochondrial electron transport chain is also known as

A · Cytochrome c oxidase
B · NADH dehydrogenase
C · Cytochrome bc1
D · Succinate dehydrogenase
Solution: Complex II is succinate dehydrogenase. It oxidises succinate to fumarate in the Krebs cycle and feeds those electrons (via FADH2) to ubiquinone, linking the Krebs cycle directly to the ETS. NADH dehydrogenase is Complex I, cytochrome bc1 is Complex III, and cytochrome c oxidase is Complex IV.

Solved Respiration in Plants NEET PYQs

Try the real previous-year questions from this chapter — each with the answer and a full solution.

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Frequently asked

What is the main function of the electron transport system?

Its main function is to make ATP. It passes electrons from NADH and FADH2 down a chain of carriers to oxygen, and the energy released is used to build a proton gradient that drives ATP synthesis. Most of the ATP from respiration is made at this stage.

What is the final electron acceptor in the ETS?

Oxygen is the final electron acceptor. It combines with electrons and protons to form water. This is why aerobic respiration cannot continue without oxygen.

Why is the ETS called oxidative phosphorylation?

Because ATP (phosphorylation) is produced using the energy of oxidation-reduction reactions in the electron transport chain. This is different from photophosphorylation, where light energy drives the proton gradient.

Which coenzyme gives more ATP, NADH or FADH2?

NADH gives more: 3 ATP per molecule versus 2 ATP for FADH2. NADH enters at Complex I and pumps more protons, while FADH2 enters later at Complex II.

Is the ETS the same in plants and animals?

Yes, the mitochondrial ETS works the same way in plant and animal cells. NCERT describes this common pathway in the Respiration in Plants chapter because plant cells respire using the same electron transport system.