What is Respiratory Quotient (RQ)?

Biology · Respiration in Plants · NEET

Respiratory Quotient (RQ) is the ratio of the volume of carbon dioxide (CO2) released to the volume of oxygen (O2) used up during respiration. Formula: RQ = volume of CO2 evolved / volume of O2 consumed. Memory hook: "RQ = Released over Required" — CO2 Released divided by O2 Required.
Respiratory Quotient (RQ)RQ formulaVolume of CO2 evolvedVolume of O2 consumed(Released over Required)RQ values by substrateCarbohydrate = 1.0Protein ≈ 0.9Fat (tripalmitin) ≈ 0.7Organic acids: RQ > 1 | Anaerobic (no O2): RQ = infinity
RQ is CO2 released divided by O2 used. Its value reveals the fuel: carbohydrate = 1, protein about 0.9, fat about 0.7, organic acids above 1.

Your doubts, answered

Is RQ CO2 over O2, or O2 over CO2? I keep mixing them up.

RQ is always CO2 evolved on top and O2 consumed on the bottom: RQ = CO2 / O2. Remember 'Released over Required' — the gas Released (CO2) is divided by the gas Required (O2). Flipping it is the most common mistake and gives a wrong answer in the exam.

Why is the RQ of carbohydrates exactly 1?

When a carbohydrate like glucose is fully oxidised, the reaction is C6H12O6 + 6O2 -> 6CO2 + 6H2O. Here 6 molecules of O2 are used and 6 molecules of CO2 are released. So RQ = 6/6 = 1. Equal gases in and out means RQ equals one.

Can RQ be greater than 1?

Yes. Organic acids like malic acid have RQ greater than 1 because they already contain a lot of oxygen, so their oxidation releases more CO2 than the O2 taken in. Fats give RQ less than 1, carbohydrates give RQ = 1, and proteins give about 0.9.

What does the RQ value actually tell us?

RQ tells us which respiratory substrate (food type) is being used. RQ = 1 points to carbohydrate, about 0.7 points to fat, and about 0.9 points to protein. So from one number you can identify the fuel the cell is burning.

Is RQ only calculated for aerobic respiration?

RQ is defined for aerobic respiration, where both O2 is consumed and CO2 is released. In pure anaerobic respiration or fermentation, no O2 is used, so RQ becomes infinite (or undefined) because you would divide by zero.

How is RQ measured in the lab?

RQ is measured using a Ganong's respirometer. It compares the CO2 given out with the O2 taken in by germinating seeds. NCERT does not ask the apparatus in detail, but it is worth knowing the ratio it measures.

⚠️ The NEET trap
RQ = volume of O2 consumed / volume of CO2 evolved
RQ = volume of CO2 evolved / volume of O2 consumed
🧠 NTA reverses the ratio in options to trap you. CO2 is always ON TOP. Say 'Released over Required' before you pick.

Real NEET questions

NEET 2019

Respiratory Quotient (RQ) value of tripalmitin is

A · 0.9
B · 0.7
C · 0.07
D · 0.09
Solution: Tripalmitin is a fat. Fats are poor in oxygen compared to carbon, so their complete oxidation uses more O2 than the CO2 released, giving RQ less than 1. NCERT works out RQ = 102/145 = 0.7 for tripalmitin. (Carbohydrate = 1, protein = about 0.9.)
NEET 2026

2(C51H98O6) + 145 O2 -> 102 CO2 + 98 H2O + energy. The Respiratory Quotient (RQ) of the biomolecule used for respiration, as per the above equation, would be:

A · Between 0.5 and 0.95
B · Less than 0.5
C · 1.0
D · Between 1.25 and 2
Solution: RQ = volume of CO2 evolved / volume of O2 consumed = 102/145 = 0.70. A value near 0.7 means a fat substrate (tripalmitin) and lies in the range 0.5 to 0.95. It is not 1.0 (that is carbohydrate) and not above 1 (that is only for organic acids).

Solved Respiration in Plants NEET PYQs

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Frequently asked

What is the respiratory quotient in simple words?

It is the ratio of CO2 gas released to O2 gas used up during respiration: RQ = CO2 / O2. It tells you which food type the cell is burning.

What is the RQ value of carbohydrates?

The RQ of carbohydrates is 1, because equal volumes of CO2 are released and O2 are consumed when a carbohydrate is fully oxidised.

What is the RQ of fats?

Fats have an RQ less than 1, about 0.7 (for example tripalmitin = 102/145 = 0.7), because their oxidation needs extra O2.

Why is RQ important for NEET?

RQ is a direct, high-frequency NEET topic. It links the substrate (carbohydrate, fat, protein) to a single number, and NEET often gives an equation and asks you to calculate or interpret the RQ.

When is RQ zero or infinite?

RQ is infinite (undefined) in anaerobic respiration where no O2 is used. It is not zero in normal respiration because CO2 is always released.