Tools of recombinant DNA technology — Biotechnology: Principles and processes NEET Questions 2026

Sample Questions — Tools of recombinant DNA technology

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MCQ

Q18. Why does cutting with restriction enzymes followed by DNA ligation constitute the core of recombinant DNA technology?

A. It allows gene therapy in humans
B. It ensures that only mRNA is cloned
C. It deletes introns
D. It enables insertion and permanent integration of foreign DNA

✅ Correct Answer: D

This basic cut-and-join technique enables stable introduction of genes into vectors.

MCQ

Assertion (A): Presence of multiple restriction sites in a cloning vector is ideal. Reason (R): Multiple sites help generate multiple fragments of foreign DNA.

A. Both A and R are true, and R is the correct explanation of A
B. Both A and R are true, but R is not the correct explanation of A
C. A is false, but R is true
D. Both A and R are false

✅ Correct Answer: C

Multiple restriction sites complicate cloning; vectors should have only one/few sites.

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MCQ

Q8. Why is the Ti plasmid considered an ideal vector for plant genetic engineering?

A. It cannot replicate in bacterial cells
B. It integrates into plant genome naturally via T-DNA
C. It suppresses expression of foreign genes
D. It amplifies the bacterial genome

✅ Correct Answer: B

Agrobacterium uses the Ti plasmid to deliver genes into dicot plant cells.

MCQ

Q5. Why does insertion of a gene into the tetracycline resistance gene of pBR322 cause loss of tetracycline resistance?

A. Insertion improves vector mobility
B. Insertion alters codons for resistance
C. Insertion disrupts coding sequence via insertional inactivation
D. Insertion induces recombination

✅ Correct Answer: C

Disrupting the antibiotic resistance gene prevents expression.

MCQ

Q2. Why must both the plasmid vector and source DNA be cut with the same restriction enzyme?

A. To prevent plasmid replication
B. To ensure sticky ends are incompatible
C. To allow complementary sticky ends for ligation
D. To make the plasmid circular

✅ Correct Answer: C

Sticky ends produced by the same enzyme pair up, enabling successful ligation.

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