Sample Questions — Processes of recombinant DNA technology
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MCQ
Which technique is used to monitor the progression of a restriction enzyme digestion?
✅ Correct Answer: D
Electrophoresis shows size-based separation of cut fragments.
MCQ
What is used to remove proteins during DNA extraction?
✅ Correct Answer: B
Proteins are removed by protease digestion.
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MCQ
What would happen if no Ca²⁺ treatment is given during transformation?
✅ Correct Answer: D
Calcium ions increase membrane permeability for DNA entry.
MCQ
Which of the following enzymes is NOT used in DNA isolation protocol?
✅ Correct Answer: C
Taq is used in PCR, not DNA isolation.
MCQ
Identify the TRUE statements:
I. Ligation is followed by transformation
II. Recombinant plasmids enter host by diffusion
III. Ca²⁺ increases cell membrane permeability
IV. DNA moves towards cathode in gel
✅ Correct Answer: A
II is false – transformation requires specific chemical/heat treatment.
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