1 How to Analyse Chemical Composition? 9.2 Primary and Secondary Metabolites 9.3 Biomacromolecules 9.4 Proteins 9.5 Polysaccharides 9.6 Nucleic Acids 9.7 Structure of Proteins 9.8 Enzymes
Which of the following statements about the chemical analysis of living tissues and the resulting fractions are correct? S1: The acid-soluble pool generally contains biomolecules with molecular weights ranging from 18 to approximately 800 Daltons. S2: Lipids are technically small molecular weight compounds, but they are found in the acid-insoluble fraction because they form water-soluble vesicles upon disruption of cell membranes. S3: Proteins, nucleic acids, and polysaccharides are examples of true biomacromolecules, typically having molecular weights above ten thousand Daltons. S4: The acid-insoluble fraction represents the entire chemical composition of the cytoplasm, while the acid-soluble pool consists primarily of inorganic constituents. S5: Grinding a living tissue in trichloroacetic acid and straining it through cheesecloth allows for the separation of the acid-soluble pool and the acid-insoluble fraction.
Correct answer: A — S1, S3 and S5 only
The question pertains to the chemical analysis of living tissues, specifically the classification of biomolecules into acid-soluble and acid-insoluble fractions based on their properties and behavior. S1: Correct. The NCERT states that compounds in the acid-soluble pool have molecular weights ranging from 18 to around 800 Daltons. S2: Incorrect. Lipids are small molecular weight compounds and are found in the acid-insoluble fraction because they form *water-insoluble* vesicles during cell disruption, not water-soluble ones. S3: Correct. Proteins, nucleic acids, and polysaccharides are true macromolecules with molecular weights typically above ten thousand Daltons, and they are polymeric substances found in the acid-insoluble fraction. S4: Incorrect. The acid-soluble pool represents roughly the cytoplasmic composition and contains thousands of organic compounds, along with inorganic constituents. The acid-insoluble fraction, along with the acid-soluble pool, represents the entire chemical composition. The statement incorrectly claims the acid-insoluble fraction represents the entire cytoplasmic composition and that the acid-soluble pool consists *primarily* of inorganic constituents. S5: Correct. The method described, grinding tissue in trichloroacetic acid and straining, is the standard procedure mentioned in NCERT for obtaining these two fractions. Therefore, statements S1, S3, and S5 are correct. The final answer is A because it accurately identifies the correct statements regarding the chemical analysis of living tissues and the properties of biomolecules.
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