Tissue Analysis: Acid-Soluble Pool — NEET Biology
📚 Practice ConceptWe can continue asking in the same way, what type of organic compounds are found in living tissues? How to analyse? Analyse a living tissue by grinding it in trichloroacetic acid using a mortar and a pestle. We can take any living tissue (a vegetable or a piece of liver, etc.) and grind it in trichloroacetic acid (Cl3CCOOH) using a mortar and a pestle. We obtain a thick slurry. If we were to strain this through a cheesecloth or cotton we would obtain two fractions. One is called the filtrate or more technically, the acid-soluble pool, and the second, the retentate or the acid-insoluble fraction. Scientists have found thousands of organic compounds in the acid-soluble pool.
Assertion (A): Tissue grinding separates cellular components into different fractions. Reason (R): The acid-insoluble fraction contains small molecules while the acid-soluble pool contains large molecules.
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