RNAi-Based Nematode Resistance — NEET Biology
📚 Practice ConceptUsing Agrobacterium vectors, nematode-specific genes were introduced into the host plant. The introduction of DNA was such that it produced both sense and anti-sense RNA in the host cells. These two RNA's being complementary to each other formed a double stranded (dsRNA) that initiated RNAi and thus, silenced the specific mRNA of the nematode. The consequence was that the parasite could not survive in a transgenic host expressing specific interfering RNA. The transgenic plant therefore got itself protected from the parasite.
Arrange the following events in the correct sequence regarding the development of RNAi-based pest-resistant plants: 1. dsRNA silences nematode mRNA, blocking translation. 2. Introduction of nematode-specific DNA via Agrobacterium vector. 3. Production of sense and antisense RNAs inside host plant cells. 4. Formation of double-stranded RNA (dsRNA). 5. Nematode fails to survive in the transgenic host.
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Correct answer: C — 2 → 3 → 4 → 1 → 5
The process begins when nematode-specific DNA is transferred into host plants by Agrobacterium. This DNA directs the production of sense and antisense RNAs, which pair to form dsRNA. The dsRNA silences nematode mRNA, preventing translation. Consequently, the nematode cannot survive in the host plant.
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