RNAi-Based Nematode Resistance — NEET Biology
📚 Practice ConceptUsing Agrobacterium vectors, nematode-specific genes were introduced into the host plant. The introduction of DNA was such that it produced both sense and anti-sense RNA in the host cells. These two RNA's being complementary to each other formed a double stranded (dsRNA) that initiated RNAi and thus, silenced the specific mRNA of the nematode. The consequence was that the parasite could not survive in a transgenic host expressing specific interfering RNA. The transgenic plant therefore got itself protected from the parasite.
Arrange the following events in the correct sequence regarding the development of RNAi-based pest-resistant plants: 1. dsRNA silences nematode mRNA, blocking translation. 2. Introduction of nematode-specific DNA via Agrobacterium vector. 3. Production of sense and antisense RNAs inside host plant cells. 4. Formation of double-stranded RNA (dsRNA). 5. Nematode fails to survive in the transgenic host.
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