Early Diagnosis Techniques in Biotechnology — NEET Biology
📚 Practice ConceptRecombinant DNA technology, Polymerase Chain Reaction (PCR) and Enzyme Linked Immuno-sorbent Assay (ELISA) are some of the techniques that serve the purpose of early diagnosis.
Which of the following statements are correct regarding molecular diagnostic techniques and their applications? S1: Polymerase Chain Reaction (PCR) can detect very low concentrations of pathogens by amplifying their nucleic acid, even when symptoms are not yet visible. S2: ELISA (Enzyme Linked Immuno-sorbent Assay) is a diagnostic technique primarily based on the principle of antigen-antibody interaction for the detection of pathogen-specific antigens or antibodies. S3: In detecting mutated genes using autoradiography, the clone having the mutated gene will appear on the photographic film because the radioactive probe forms a stable hybrid with the mutated sequence. S4: PCR is routinely employed for the detection of HIV in suspected AIDS patients and for identifying mutations in genes in suspected cancer patients, making it a powerful technique for genetic disorders. S5: Enzyme replacement therapy is highlighted in NCERT as a completely curative approach for adenosine deaminase (ADA) deficiency, unlike gene therapy which offers only temporary solutions.
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