Gene Probe Hybridization for Mutation Detection — NEET Biology
📚 Practice Concept📖 NCERT Source
A single stranded DNA or RNA, tagged with a radioactive molecule (probe) is allowed to hybridise to its complementary DNA in a clone of cells followed by detection using autoradiography. The clone having the mutated gene will hence not appear on the photographic film, because the probe will not have complementarity with the mutated gene.
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QuestionPractice Question
Assertion (A): Genetic disorders can be detected using molecular probes. Reason (R): Radioactive DNA probes hybridise to complementary sequences and are detected by autoradiography.
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