Biotechnology has given to humans several useful products by using microbes, plant, animals and their metabolic machinery. Techniques of tissue culture and somatic hybridisation offer vast potential for manipulation of plants in vitro to produce new varieties. Recombinant DNA technology has made it possible to engineer microbes, plants and animals such that they have novel capabilities. Genetically Modified Organisms have been created by using methods other than natural methods to transfer one or more genes from one organism to another, generally using techniques such as recombinant DNA technology.
Which of the above statements regarding plant tissue culture techniques are correct? S1: Meristem culture is utilized for obtaining virus-free plants because the apical and axillary meristems are generally free of viral infection. S2: Micropropagation is the method of producing a large number of genetically identical plants, referred to as somaclones, in a short duration. S3: The nutrient medium for in vitro plant culture typically contains only inorganic salts and a carbon source, without the need for growth regulators. S4: Somatic hybridisation involves the fusion of protoplasts from two different plant varieties to produce a new hybrid plant. S5: Pomato is a commercially highly successful somatic hybrid plant extensively used in agriculture.
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