Plant Tissue Culture Medium Components — NEET Biology

Correct answer: A — a-i, b-ii, c-iv, d-iii, e-v

Let's match the items from Column I with Column II: a. Micro-propagation: This method produces thousands of plants through tissue culture, and these plants are genetically identical to the original plant, called somaclones. So, a-i is correct. b. Somatic hybridisation: This process involves isolating naked protoplasts from different varieties of plants and fusing them to get hybrid protoplasts, which can then be grown to form a new plant. So, b-ii is correct. c. Agrobacterium tumefaciens: This bacterium is known as a natural genetic engineer for plants because it can transfer a piece of DNA (T-DNA) into plant cells. It is widely used as a vector for gene transfer in plants. So, c-iv is correct. d. RNA interference (RNAi): This is a method of cellular defense involving the silencing of a specific mRNA due to a complementary dsRNA molecule. In pest-resistant plants, it silences specific nematode mRNA. So, d-iii is correct. e. Bt toxin gene: This gene, cloned from Bacillus thuringiensis, is expressed in plants to produce insecticidal proteins (Bt toxin), providing resistance to insects. So, e-v is correct. Based on these matches, option A is the correct answer.