Recombinant DNA & Gene Expression in Bacteria — NEET Biology
📚 Practice ConceptDigrammatically represent the experimental steps in cloning and expressing an human gene (say the gene for growth hormone) into a bacterium like E. coli?
Arrange the following steps in the correct chronological order as they occur during the natural synthesis and maturation of human insulin, and its production using recombinant DNA technology: 1. Formation of disulfide bridges between A and B chains. 2. Synthesis of pro-hormone (pro-insulin) with C-peptide. 3. Introduction of DNA sequences for A and B chains into E. coli plasmids. 4. Separate production and extraction of A and B polypeptide chains in bacteria. 5. Enzymatic removal of C-peptide from pro-insulin. 6. Combination of separately produced A and B chains by creating disulfide bonds in vitro.
Through deep analysis of NEET and NTA, 88 of 90 questions from the NEET 2026 paper were matched straight from the MedicNEET Biology question bank.
88/90MedicNEET's Biology question bank is built from the same NCERT lines NTA picks repeatedly. Not random MCQs — questions crafted exactly like NTA crafts them.