Steps of Gene Transfer — NEET Biology
🔥 Predicted for NEET 2027Identification of DNA with desirable genes; (ii) introduction of the identified DNA into the host; (iii) maintenance of introduced DNA in the host and transfer of the DNA to its progeny.
Adjacent to a frequently-asked paragraph in the same chapter.
A scientist is cloning a human growth hormone gene. Which integrated steps are correct? 1. Identification of gene from human genome 2. Cutting DNA with EcoRI and ligating into pBR322 plasmid 3. Transforming competent E. coli using calcium chloride and heat shock 4. Growing recombinant E. coli in a stirred tank bioreactor
View solution & NCERT explanation
Correct answer: D — 1, 2, 3 and 4
To obtain recombinant growth hormone: The gene must first be identified and isolated from the human genome. It is then cut with EcoRI and ligated into a cloning vector like pBR322, which ensures replication via its ori. Competent E. coli cells treated with calcium chloride and heat shock allow uptake of this recombinant plasmid. Finally, to yield usable hormone at commercial scale, the transformed E. coli is cultured in a bioreactor under controlled aeration, pH, and temperature.
What does NCERT say about Identification DNA with desirable?
Has this concept appeared in NEET?
Which chapter is this from?
Through deep analysis of NEET and NTA, 176 of the 180 ReNEET 2026 (June 21) questions were already in the MedicNEET question bank before the exam.
176/180MedicNEET's Biology question bank is built from the same NCERT lines NTA picks repeatedly. Not random MCQs — questions crafted exactly like NTA crafts them.