Identification of DNA with desirable genes; (ii) introduction of the identified DNA into the host; (iii) maintenance of introduced DNA in the host and transfer of the DNA to its progeny.
Adjacent to a frequently-asked paragraph in the same chapter.
A scientist is cloning a human growth hormone gene. Which integrated steps are correct? 1. Identification of gene from human genome 2. Cutting DNA with EcoRI and ligating into pBR322 plasmid 3. Transforming competent E. coli using calcium chloride and heat shock 4. Growing recombinant E. coli in a stirred tank bioreactor
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