Gene Expression in Recombinant DNA — NEET Biology
🔥 Predicted for NEET 2027When you insert a piece of alien DNA into a cloning vector and transfer it into a bacterial, plant or animal cell, the alien DNA gets multiplied. In almost all recombinant technologies, the ultimate aim is to produce a desirable protein. Hence, there is a need for the recombinant DNA to be expressed. The foreign gene gets expressed under appropriate conditions. The expression of foreign genes in host cells involve understanding many technical details.
Adjacent to a frequently-asked paragraph in the same chapter.
Arrange the following steps involved in obtaining a foreign gene product through recombinant DNA technology in the correct sequence: 1. Insertion of alien DNA into a suitable cloning vector and its transfer into a host cell 2. Expression of the foreign gene under appropriate conditions to produce a target protein 3. Large-scale culture of recombinant host cells using bioreactors under optimal conditions 4. Extraction and purification of the desired protein using separation techniques 5. Multiplication of recombinant DNA inside the host cells
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