Gene Expression in Recombinant DNA — NEET Biology
🔥 Predicted for NEET 2027When you insert a piece of alien DNA into a cloning vector and transfer it into a bacterial, plant or animal cell, the alien DNA gets multiplied. In almost all recombinant technologies, the ultimate aim is to produce a desirable protein. Hence, there is a need for the recombinant DNA to be expressed. The foreign gene gets expressed under appropriate conditions. The expression of foreign genes in host cells involve understanding many technical details.
Adjacent to a frequently-asked paragraph in the same chapter.
Arrange the following steps involved in obtaining a foreign gene product through recombinant DNA technology in the correct sequence: 1. Insertion of alien DNA into a suitable cloning vector and its transfer into a host cell 2. Expression of the foreign gene under appropriate conditions to produce a target protein 3. Large-scale culture of recombinant host cells using bioreactors under optimal conditions 4. Extraction and purification of the desired protein using separation techniques 5. Multiplication of recombinant DNA inside the host cells
View solution & NCERT explanation
Correct answer: D — 1 → 5 → 2 → 3 → 4
Alien DNA is first inserted into a vector and transferred into a host cell. Inside the host, the recombinant DNA multiplies. The foreign gene is then expressed under suitable conditions to form the desired protein. For commercial purposes, host cells are cultured in large quantities using bioreactors, which provide optimal growth conditions. Finally, the protein is extracted and purified.
What does NCERT say about When you insert piece?
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Through deep analysis of NEET and NTA, 176 of the 180 ReNEET 2026 (June 21) questions were already in the MedicNEET question bank before the exam.
176/180MedicNEET's Biology question bank is built from the same NCERT lines NTA picks repeatedly. Not random MCQs — questions crafted exactly like NTA crafts them.