Recombinant Protein Production & Scale-Up — NEET Biology
📚 Practice ConceptAfter having cloned the gene of interest and having optimised the conditions to induce the expression of the target protein, one has to consider producing it on a large scale. Can you think of any reason why there is a need for large-scale production? If any protein encoding gene is expressed in a heterologous host, it is called a recombinant protein. The cells harbouring cloned genes of interest may be grown on a small scale in the laboratory. The cultures may be used for extracting the desired protein and then purifying it by using different separation techniques.
Arrange the following steps involved in obtaining a foreign gene product through recombinant DNA technology in the correct sequence: 1. Insertion of alien DNA into a suitable cloning vector and its transfer into a host cell 2. Expression of the foreign gene under appropriate conditions to produce a target protein 3. Large-scale culture of recombinant host cells using bioreactors under optimal conditions 4. Extraction and purification of the desired protein using separation techniques 5. Multiplication of recombinant DNA inside the host cells
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