Active Log Phase & Biomass Production — NEET Biology
📚 Practice ConceptThis type of culturing method produces a larger biomass leading to higher yields of desired protein.
Which of the following statements regarding recombinant protein production are NOT correct? S1: The process of genetic engineering invariably starts with the isolation of the target DNA fragment from the source organism. S2: Transformation is the process where a piece of DNA is introduced into a host bacterium to create recombinant cells. S3: Bioreactors provide optimal growth conditions for culturing recombinant host cells on a large scale (100-1000 litres) for maximum product yield. S4: Gram staining is a routinely employed method to select transformants containing the recombinant DNA from non-transformants. S5: Downstream processing is carried out prior to the biosynthetic stage to ensure quality control and product formulation. S6: Bioprocess engineering focuses on maintaining sterile ambience to allow the growth of only the desired microbe/eukaryotic cell in large quantities.
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