Restriction Enzymes & DNA Cutting Sites — NEET Biology
📚 Practice ConceptMake a chart (with diagrammatic representation) showing a restriction enzyme, the substrate DNA on which it acts, the site at which it cuts DNA and the product it produces.
Which of the following statements are correct regarding blue-white screening for identifying recombinant clones? S1: Blue-white screening relies on the insertional inactivation of the lacZ gene in the presence of a foreign DNA insert. S2: Non-recombinant colonies produce a white colour because they lack a functional β-galactosidase enzyme. S3: The chromogenic substrate, X-gal, is cleaved by β-galactosidase to produce a blue product. S4: Successful recombinant clones are identified as white colonies due to the absence of β-galactosidase activity. S5: This method is less cumbersome compared to the selection based on antibiotic resistance for differentiating recombinants.
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