Do eukaryotic cells have restriction endonucleases? Justify your answer.
Arrange the following steps in the correct sequential order as they occur during the process of recombinant DNA technology: S1: Isolation of the genetic material (DNA). S2: Cutting of DNA at specific locations using restriction endonucleases. S3: Separation and isolation of the desired DNA fragment. S4: Ligation of the DNA fragment into a suitable vector. S5: Insertion of the recombinant DNA into a competent host cell. S6: Culturing the host cells in a medium at large scale.
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