Class 12 · Biotechnology: Principles and Processes

Stirred Tank Bioreactors: Design & Advantages — NEET Biology

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Besides better aeration and mixing properties, what other advantages do stirred tank bioreactors have over shake flasks?

NCERT Biology · Class 12 · Chapter 9 · Paragraph 64
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Which of the following statements regarding bioreactors and downstream processing are correct? S1: Bioreactors are essential for producing recombinant proteins on a large scale (100-1000 litres). S2: The primary function of a bioreactor is to provide sub-optimal conditions for maximum growth of the desired organism. S3: A stirred-tank bioreactor typically has a flat base to prevent mixing of reactor contents. S4: The agitator system, oxygen delivery system, and pH control system are standard features of a bioreactor. S5: Downstream processing involves the separation and purification of the desired product after the biosynthetic stage.

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Correct answer: B S1, S4 and S5 are correct

The question asks for correct statements about bioreactors and downstream processing. S1 is correct: Bioreactors are indeed used for large-scale production, processing volumes typically from 100-1000 litres. S2 is incorrect: Bioreactors are designed to provide *optimal* growth conditions, not sub-optimal, to achieve the desired product. S3 is incorrect: A stirred-tank reactor usually has a *curved* base to facilitate even mixing of contents, not a flat base to prevent mixing. S4 is correct: Standard features of a bioreactor include an agitator system (for mixing), an oxygen delivery system, and pH and temperature control systems. S5 is correct: Downstream processing is the series of steps (separation and purification) that follow the biosynthetic stage to prepare the product for marketing. Therefore, statements S1, S4, and S5 are correct. Final Answer: The correct statements are S1, S4, and S5.

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Besides better aeration and mixing properties, what other advantages do stirred tank bioreactors have over shake flasks?
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Biotechnology: Principles and Processes, Class 12 NCERT Biology.

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