Palindromic DNA Sequences — NEET Biology
📚 Practice ConceptCollect 5 examples of palindromic DNA sequences by consulting your teacher. Better try to create a palindromic sequence by following base-pair rules.
Which of the following integrated statements are true? 1. Restriction endonuclease recognises palindromic sequences and produces sticky ends . 2. Ligase seals sticky ends and allows plasmid insertion 3. Competent bacterial cells take up recombinant plasmid through transformation . 4. Downstream processing begins before recombinant protein expression .
View solution & NCERT explanation
Correct answer: B — 1, 2 and 3 only
Restriction enzymes such as EcoRI cut at palindromic sequences, leaving sticky ends. DNA ligase seals these sticky ends, enabling stable recombinant plasmid formation (Tool + Process). Competent E. coli then internalises the recombinant plasmid during transformation via calcium chloride + heat shock. However, downstream processing refers to purification and formulation of the final protein after expression, not before. Hence, only statements 1, 2, and 3 are correct.
What does NCERT say about Collect 5 examples palindromic?
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Through deep analysis of NEET and NTA, 176 of the 180 ReNEET 2026 (June 21) questions were already in the MedicNEET question bank before the exam.
176/180MedicNEET's Biology question bank is built from the same NCERT lines NTA picks repeatedly. Not random MCQs — questions crafted exactly like NTA crafts them.