Reporter Enzymes in Genetic Transformation — NEET Biology
📚 Practice ConceptCan you think and answer how a reporter enzyme can be used to monitor transformation of host cells by foreign DNA in addition to a selectable marker?
Which of the following statements are correct regarding cloning vectors and their characteristics? S1: Plasmids are always preferred over bacteriophages for cloning genes that require very high copy numbers within the host cell. S2: The origin of replication (ori) sequence is responsible for initiating replication and controlling the copy number of the linked DNA. S3: Selectable markers help in identifying transformants and eliminating non-transformants from a bacterial population. S4: The pBR322 vector has a single recognition site for each of its restriction enzymes within its antibiotic resistance genes, facilitating easy insertion of foreign DNA. S5: Insertional inactivation of a selectable marker gene, such as β-galactosidase, results in blue-coloured colonies in the presence of a chromogenic substrate.
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