Describe briefly the following: (a) Origin of replication (b) Bioreactors (c) Downstream processing
Which of the following integrated statements are correct regarding recombinant DNA construction? 1. Linking a gene with origin of replication ensures multiplication in host cells . 2. Restriction enzymes cut DNA at random non-specific sites . 3. Ligase joins foreign DNA to plasmid vector 4. Transformation requires competent host cells, often prepared by CaCl₂ and heat shock . 5. Downstream processing refers to separation and purification of recombinant protein .
Correct answer: C — 1, 3, 4, 5
Restriction enzymes cut DNA at specific palindromic sites, not random ones. All other steps (ori-linked replication, ligase action, transformation, and downstream purification) are correct integrations of principles, tools, and processes.
MedicNEET's Biology question bank is built from the same NCERT lines NTA picks repeatedly. Not random MCQs — questions crafted exactly like NTA crafts them.