Class 12 · Biotechnology: Principles and Processes

Biotechnology Process: Key Concepts — NEET Biology

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Describe briefly the following: (a) Origin of replication (b) Bioreactors (c) Downstream processing

NCERT Biology · Class 12 · Chapter 9 · Paragraph 68
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Which of the following integrated statements are correct regarding recombinant DNA construction? 1. Linking a gene with origin of replication ensures multiplication in host cells . 2. Restriction enzymes cut DNA at random non-specific sites . 3. Ligase joins foreign DNA to plasmid vector 4. Transformation requires competent host cells, often prepared by CaCl₂ and heat shock . 5. Downstream processing refers to separation and purification of recombinant protein .

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Correct answer: C 1, 3, 4, 5

Restriction enzymes cut DNA at specific palindromic sites, not random ones. All other steps (ori-linked replication, ligase action, transformation, and downstream purification) are correct integrations of principles, tools, and processes.

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Describe briefly the following: (a) Origin of replication (b) Bioreactors (c) Downstream processing
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Biotechnology: Principles and Processes, Class 12 NCERT Biology.

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