Describe briefly the following: (a) Origin of replication (b) Bioreactors (c) Downstream processing
Which of the following integrated statements are correct regarding recombinant DNA construction? 1. Linking a gene with origin of replication ensures multiplication in host cells . 2. Restriction enzymes cut DNA at random non-specific sites . 3. Ligase joins foreign DNA to plasmid vector 4. Transformation requires competent host cells, often prepared by CaCl₂ and heat shock . 5. Downstream processing refers to separation and purification of recombinant protein .
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