Explain briefly (a) PCR (b) Restriction enzymes and DNA (c) Chitinase
Assertion (A): Restriction enzymes create staggered cuts in DNA. Reason (R): Restriction enzymes cut exactly at the center of palindromic recognition sequences.
Correct answer: C — If assertion is true but reason is false.
NCERT confirms restriction enzymes create staggered cuts producing sticky ends, making the Assertion true. However, NCERT explicitly states “Restriction enzymes cut the strand of DNA a little away from the centre of the palindrome sites, but between the same two bases on the opposite strands.” The Reason falsely claims cutting at exact center - cutting away from center creates the staggered ends.
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