The two chains have anti-parallel polarity. It means, if one chain has the polarity 5'→3', the other has 3'→5'.
Adjacent to a frequently-asked paragraph in the same chapter.
What feature of the DNA strands’ polarity is crucial for the structure of the double helix?
Correct answer: C — Both strands are antiparallel, one 5'→3' and the other 3'→5'
The antiparallel nature allows complementary base pairing and helix formation.
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