DNA Fingerprinting & VNTR Technique — NEET Biology
✅ Asked in NEET 2024The technique of DNA Fingerprinting was initially developed by Alec Jeffreys. He used a satellite DNA as probe that shows very high degree of polymorphism. It was called as Variable Number of Tandem Repeats (VNTR). The technique, as used earlier, involved Southern blot hybridisation using radiolabelled VNTR as a probe. It included
DNA fingerprinting, developed by Alec Jeffreys, uses Variable Number of Tandem Repeats (VNTR) as probes to identify individuals based on unique DNA patterns. VNTRs are satellite DNA sequences showing high polymorphism, making each person's fingerprint unique. Students often confuse VNTR with other repetitive DNA or forget that satellite DNA with high polymorphism is specifically used. The key point to remember: VNTRs are highly polymorphic tandem repeats that form the basis of DNA fingerprinting through Southern blot hybridization. NEET may test whether you understand why VNTRs are ideal for fingerprinting—their variability makes discrimination between individuals highly reliable, unlike low-polymorphism markers.
Match List-I with List-II: (NEET 2024) List-I: A. DNA Fingerprinting B. Transformation C. Semiconservative replication D. DNA as genetic material List-II: I. E. coli II. Bacteriophage III. Pneumococcus IV. Alec Jeffreys Choose the correct answer from the options given below:
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