We need to emphasise here that the water splitting complex is associated with the PS II, which itself is physically located on the inner side of the membrane of the thylakoid. Then, where are the protons and O2 formed likely to be released – in the lumen? or on the outer side of the membrane?
Which of the following statements accurately describe the process of water splitting and oxygen evolution in photosynthesis? S1: Oxygen evolution in higher plants is directly associated with Photosystem I (PSI). S2: The water-splitting complex is physically located on the inner side of the thylakoid membrane. S3: Electrons released from water splitting replenish those lost by PSII, maintaining continuous electron transport. S4: Protons generated during water splitting are released into the thylakoid lumen, contributing to the proton gradient. S5: The oxygen released during photosynthesis originates from carbon dioxide molecules.
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