What Are Enzymes? Proteins vs Ribozymes

Biology · Biomolecules · NEET

An enzyme is a biocatalyst. It speeds up a chemical reaction inside the cell but is not used up. NCERT says "almost all enzymes are proteins." A few enzymes are made of nucleic acid (RNA), not protein. These RNA enzymes are called ribozymes. Memory hook: "almost ALL, not ALL" — the exception is the ribozyme (Ribo = RNA).
Enzymes = BiocatalystsENZYMESProtein enzymes"Almost all" enzymesmade of amino acidsRibozymesNon-protein enzymesmade of RNA (nucleic acid)Both speed up reactions and lower activation energy
Enzymes are biocatalysts. Almost all are protein enzymes (made of amino acids); the exception is ribozymes, which are non-protein enzymes made of RNA. Both act as biocatalysts.

Your doubts, answered

Are all enzymes proteins?

No. This is the exact trap NEET uses. NCERT says 'almost all enzymes are proteins' — not ALL. Most enzymes are made of protein, but some nucleic acids (RNA) also act as enzymes. So the correct statement is 'almost all enzymes are proteins,' and any option that says 'all enzymes are proteins' is wrong.

What is a ribozyme?

A ribozyme is a nucleic acid (RNA) that behaves like an enzyme. In NCERT words: 'There are some nucleic acids that behave like enzymes. These are called ribozymes.' So a ribozyme is a non-protein enzyme. It is made of RNA, not amino acids.

Is a ribozyme a protein or a nucleic acid?

A ribozyme is a nucleic acid (RNA). This is the point NEET tests. When a question asks for a 'non-proteinaceous enzyme,' the answer is ribozyme. Options like lysozyme, ligase and deoxyribonuclease are all proteins, so they are wrong.

What is the difference between an enzyme and a ribozyme?

An enzyme is the broad group — any biocatalyst that speeds up a reaction. Most enzymes are proteins (chains of amino acids). A ribozyme is a special kind of enzyme made of RNA instead of protein. So a ribozyme IS an enzyme, but a non-protein one. Remember: all ribozymes are enzymes, but not all enzymes are ribozymes.

Do enzymes have the same structure as other proteins?

Yes, protein enzymes follow the same 4 levels of protein structure. NCERT says an enzyme, like any protein, has a primary structure (amino acid sequence), a secondary structure and a tertiary structure. When the chain folds, it forms pockets. One such pocket is the active site where the substrate fits.

What is the most abundant enzyme in the world?

RuBisCO (Ribulose bisphosphate Carboxylase-Oxygenase) is the most abundant enzyme and most abundant protein in the whole biosphere. This is a direct NCERT fact and a repeated NEET point. Do not confuse it with collagen, which is the most abundant protein only in the animal world.

⚠️ The NEET trap
All enzymes are proteins.
Almost all enzymes are proteins; some nucleic acids (RNA) also act as enzymes and are called ribozymes.
🧠 NEET writes 'almost all enzymes are proteins' as a correct statement (2026). If you memorised 'all enzymes are proteins,' you will mark it wrong. Keep the word ALMOST — the ribozyme is the exception.

Real NEET questions

2016

A non-proteinaceous enzyme is:

A · Lysozyme
B · Ribozyme
C · Ligase
D · Deoxyribonuclease
Solution: Almost all enzymes are proteins, but some nucleic acids (RNA) also act as biocatalysts; these non-protein enzymes are called ribozymes. Lysozyme, ligase and deoxyribonuclease are all proteins, so only the ribozyme is non-proteinaceous. NCERT: 'There are some nucleic acids that behave like enzymes. These are called ribozymes.'
2026

Identify the correct statements about biomolecules: A. Lipids are generally water soluble. B. Proteins are polypeptides. C. Polysaccharides are long chains of sugars. D. Adenine and guanine are substituted pyrimidines. E. Almost all enzymes are proteins.

A · B, D and E only
B · B, C and E only
C · A, B and C only
D · C, D and E only
Solution: A is wrong (lipids are water insoluble). D is wrong (adenine and guanine are substituted purines, not pyrimidines). B, C and E are all direct NCERT statements — proteins are polypeptides, polysaccharides are long chains of sugars, and 'almost all enzymes are proteins.' Note NCERT says ALMOST all, allowing for ribozymes.

Solved Biomolecules NEET PYQs

Try the real previous-year questions from this chapter — each with the answer and a full solution.

See all 51 Biomolecules NEET PYQs ›
Next concept: Active Site and Enzyme Substrate SpecificityKeep learning — 2 minFeeling ready? Solve the Biomolecules NEET PYQs ›Or practice on your phone — get the free MedicNEET app ›

Frequently asked

Are enzymes biomolecules?

Yes. Enzymes are biomolecules. Protein enzymes belong to the protein group and ribozymes belong to the nucleic acid group. All enzymes are biocatalysts made by living cells.

Why are almost all enzymes proteins and not all?

Most reactions in cells are sped up by proteins with an active site. But a few RNA molecules can also fold into a shape that speeds up a reaction. Because these RNA molecules exist, NCERT carefully writes 'almost all' instead of 'all.' The RNA enzymes are ribozymes.

Is ribozyme made of DNA or RNA?

A ribozyme is made of RNA. It is a ribonucleic acid with catalytic power. NCERT groups ribozymes as nucleic acids that behave like enzymes.

What is the main job of an enzyme?

An enzyme lowers the activation energy of a reaction and speeds it up greatly, without being used up. For example, carbonic anhydrase speeds up the making of carbonic acid about 10 million times.

Do ribozymes have an active site like protein enzymes?

Ribozymes also fold into a 3D shape and bind their substrate to catalyse a reaction, similar in principle to protein enzymes. For NEET, the key fact is simply that ribozymes are non-protein (RNA) enzymes.