Difference Between C3 and C4 Plants

Biology · Photosynthesis in Higher Plants · NEET

C3 plants make a 3-carbon acid (PGA) as the first product using RuBP as the CO2 acceptor, have no Kranz anatomy, and show photorespiration. C4 plants make a 4-carbon acid (OAA) first using PEP as the acceptor, have Kranz anatomy, and do NOT show photorespiration. Memory hook: the number in "C3" and "C4" is the carbon count of the first stable product.
C3 vs C4 PlantsC3 PlantsC4 PlantsAcceptor: RuBP (5-C)Enzyme: RuBisCO1st product: PGA (3-C)No Kranz anatomyPhotorespiration: YESAcceptor: PEP (3-C)Enzyme: PEPcase1st product: OAA (4-C)Kranz anatomy: YESPhotorespiration: NO
Side-by-side comparison: C3 plants use RuBP/RuBisCO to make 3-carbon PGA and show photorespiration; C4 plants use PEP/PEPcase to make 4-carbon OAA, have Kranz anatomy, and avoid photorespiration.

Your doubts, answered

Does the number in C3 and C4 mean the number of carbons in the first stable product?

Yes. In C3 plants the first stable product of CO2 fixation is 3-phosphoglyceric acid (PGA), a 3-carbon acid, so the pathway is called C3. In C4 plants the first stable product is oxaloacetic acid (OAA), a 4-carbon acid, so it is called C4. This one idea unlocks most of the differences, so learn it first.

Which enzyme and which acceptor fixes CO2 first in C3 vs C4 plants?

In C3 plants CO2 is fixed by RuBisCO onto the 5-carbon acceptor RuBP. In C4 plants CO2 is first fixed by PEP carboxylase (PEPcase) onto the 3-carbon acceptor PEP in the mesophyll cells. Note: C4 mesophyll cells do not have RuBisCO; RuBisCO works only in the bundle sheath cells of C4 plants.

Do both C3 and C4 plants use the Calvin cycle?

Yes, both use the Calvin (C3) cycle as the main biosynthetic pathway that actually makes sugar. In C3 plants the Calvin cycle runs in the mesophyll. In C4 plants the C4 (Hatch and Slack) pathway just concentrates CO2, and the Calvin cycle runs only in the bundle sheath cells. So the Calvin cycle is NOT the difference between them.

Why do C4 plants not show photorespiration but C3 plants do?

In C3 plants, when O2 is high and CO2 is low, O2 binds RuBisCO and starts photorespiration, wasting fixed carbon. C4 plants use their C4 pathway to pump CO2 into the bundle sheath cells, keeping CO2 very high around RuBisCO. High CO2 makes RuBisCO carboxylate (not oxygenate), so photorespiration does not occur. This is why NCERT calls photorespiration the process that makes the major difference between C3 and C4 plants.

What is Kranz anatomy and which type has it?

Kranz anatomy is a leaf structure where large bundle sheath cells, rich in chloroplasts, form a ring (wreath) around the vascular bundles. Only C4 plants have Kranz anatomy; C3 plants do not. The bundle sheath cells have thick walls, no intercellular spaces, and hold the Calvin cycle in C4 plants.

Which plants are C3 and which are C4?

C3 examples: rice, wheat, and most temperate plants. C4 examples: maize (corn), sugarcane, sorghum, and many plants of dry tropical regions. A quick tip: sorghum and maize appear in PYQs as C4, so their first product is OAA, not PGA.

⚠️ The NEET trap
Thinking the Calvin cycle is what makes C3 and C4 plants different.
Both C3 and C4 plants run the Calvin cycle; the process that makes the major difference is photorespiration (present in C3, absent in C4).
🧠 Calvin cycle = common to both. Photorespiration = the real divider.

Real NEET questions

2016

The process which makes major difference between C3 and C4 plants is

A · Glycolysis
B · Calvin cycle
C · Photorespiration
D · Respiration
Solution: NCERT identifies photorespiration as the process that creates the important difference between C3 and C4 plants: it occurs in C3 plants (O2 binds RuBisCO) but is absent in C4 plants because their CO2-concentrating mechanism keeps RuBisCO carboxylating. The Calvin cycle runs in BOTH, so it cannot be the distinguishing process. Answer: Photorespiration.
2016

A plant in your garden avoids photorespiratory losses, has improved water use efficiency, shows high rates of photosynthesis at high temperatures and has improved efficiency of nitrogen utilization. In which of the following physiological groups would you assign this plant?

A · C3
B · C4
C · CAM
D · Nitrogen fixer
Solution: A plant that lacks photorespiration, tolerates high temperatures, and uses water and nitrogen efficiently is a C4 plant. NCERT states C4 plants tolerate higher temperatures, respond to high light intensities, lack photorespiration and have greater biomass productivity. C3 plants show photorespiratory losses, so the answer is C4.
2022

Statement I: The primary CO2 acceptor in C4 plants is phosphoenolpyruvate and is found in the mesophyll cells. Statement II: Mesophyll cells of C4 plants lack RuBisCo enzyme. Choose the correct answer.

A · Both Statement I and Statement II are correct
B · Both Statement I and Statement II are incorrect
C · Statement I is correct but Statement II is incorrect
D · Statement I is incorrect but Statement II is correct
Solution: Statement I is correct: the primary CO2 acceptor in C4 plants is PEP, a 3-carbon molecule in the mesophyll cells. Statement II is also correct: NCERT states the mesophyll cells lack RuBisCO (it is confined to the bundle sheath cells). Both statements are correct.

Solved Photosynthesis in Higher Plants NEET PYQs

Try the real previous-year questions from this chapter — each with the answer and a full solution.

See all 32 Photosynthesis in Higher Plants NEET PYQs ›
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Frequently asked

What is the main difference between C3 and C4 plants?

The first stable product: C3 plants make a 3-carbon acid (PGA), C4 plants make a 4-carbon acid (OAA). The process that makes the major difference is photorespiration, which happens in C3 plants but not in C4 plants.

What is the first stable product in C3 and C4 plants?

In C3 plants the first stable product is 3-phosphoglyceric acid (PGA), a 3-carbon acid. In C4 plants it is oxaloacetic acid (OAA), a 4-carbon acid.

Which plants have Kranz anatomy, C3 or C4?

Only C4 plants have Kranz anatomy, with large chloroplast-rich bundle sheath cells arranged in a ring around the vascular bundles. C3 plants do not have Kranz anatomy.

Do C4 plants use the Calvin cycle?

Yes. C4 plants use the C4 (Hatch and Slack) pathway to concentrate CO2, but the Calvin cycle is still the main sugar-making pathway, running only in the bundle sheath cells.

Give examples of C3 and C4 plants.

C3: rice, wheat. C4: maize (corn), sugarcane, sorghum. C4 plants are common in dry tropical regions.